Quantification and immunolocalisation of porcine articular and growth plate cartilage collagens
| Autor: | V.C. Duance, R.J. Wardale |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cartilage
Articular Molecular Structure Swine Cartilage Fluorescent Antibody Technique Cell Biology Anatomy Articular surface Matrix (biology) Biology Skeleton (computer programming) Molecular biology Immunohistochemistry Collagen type I alpha 1 medicine.anatomical_structure medicine Perichondrium Animals Collagen Growth Plate Type I collagen Growth plate cartilage |
| Zdroj: | Journal of cell science. 105 |
| ISSN: | 0021-9533 |
| Popis: | The collagens of growth plate and articular cartilage from 5–6 month old commercial pigs were characterised. Growth plate cartilage was found to contain less total collagen than articular cartilage as a proportion of the dry weight. Collagen types I, II, VI, IX and XI are present in both growth plate and articular cartilage whereas type X is found exclusively in growth plate cartilage. Types III and V collagen could not be detected in either cartilage. Type I collagen makes up at least 10% of the collagenous component of both cartilages. There are significant differences in the ratios of the quantifiable collagen types between growth plate and articular cartilage. Collagen types I, II, and XI were less readily extracted from growth plate than from articular cartilage following pepsin treatment, although growth plate cartilage contains less of the mature collagen cross-links, hydroxylysyl-pyridinoline and lysyl-pyridinoline. Both cartilages contain significant amounts of the divalent reducible collagen cross-links, hydroxylysyl-ketonorleucine and dehydro-hydroxylysinonorleucine. Immunofluorescent localisation indicated that type I collagen is located predominantly at the surface of articular cartilage but is distributed throughout the matrix in growth plate. Types II and XI are located in the matrix of both cartilages whereas type IX is predominantly pericellular in the calcifying region of articular cartilage and the hypertrophic region of the growth plate. Collagen type VI is located primarily as a diffuse area at the articular surface. |
| Databáze: | OpenAIRE |
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