Stem cells in end-to-side neurorrhaphy. Experimental study in rats
| Autor: | Geruza Rezende Paiva, Maria Aparecida Domingues Custódio, Elenice Deffune, Fausto Viterbo |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microsurgery
medicine.diagnostic_test RD1-811 business.industry Stem Cells Mesenchymal stem cell Adipose tissue Fascia Electromyography Anatomy Nerve Regeneration Rats 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure 030220 oncology & carcinogenesis medicine 030211 gastroenterology & hepatology Surgery Peripheral Nerves Stem cell business Tibial nerve Common peroneal nerve Reinnervation |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Acta Cirúrgica Brasileira, Volume: 35, Issue: 12, Article number: e351207, Published: 20 JAN 2021 Acta Cirurgica Brasileira, Vol 35, Iss 12 (2021) Acta Cirúrgica Brasileira v.35 n.12 2020 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
| ISSN: | 0102-8650 |
| Popis: | Made available in DSpace on 2021-06-25T12:34:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01. Added 1 bitstream(s) on 2021-07-15T15:06:13Z : No. of bitstreams: 1 S0102-86502020001200206.pdf: 4434117 bytes, checksum: 48a1770bcebdc3b89ddb570867be8d60 (MD5) Purpose: To evaluate the influence of mesenchymal stem cells from adipose tissue in the end-to-side neurorrhaphy, focusing in the nerve regeneration and the muscle reinnervation in acute trauma. Methods: 140 animals were randomly divided in seven groups: control, denervated, end-to-side neurorrhaphy between distal stump of common peroneal nerve and tibial nerve (ESN), ESN wrapped in fascia, ESN wrapped in fascia and platelet gel, ESN wrapped in platelet gel, ESN wrapped in fascia and platelet gel within stem cells (without culture) removed from the adipose tissue. Mass measurements of the animal and of cranial tibial muscles, electromyography, walking track analysis tests and histological examinations of the nerves and muscles after 180 days was performed. Results: In the groups where the ESN was performed, the results were always better when compared to the denervated group, showing reinnervation in all ESN groups. The most sensitive methods were walking track and histological analysis. Only the group with stem cells showed values similar to the control group, as well as the functional indices of peroneal nerve and the number of nerve fibers in the peroneal nerve. Conclusion: Stem cells were effective in ESN according with the functional index of the peroneal nerve, evaluated by walking track analysis and the number of nerve fibers in the peroneal nerve. Univ Estadual Paulista, Postgrad Program Gen Basis Surg, Botucatu, SP, Brazil Univ Estadual Paulista, Botucatu Med Sch, Plast Surg Div, Botucatu, SP, Brazil Univ Estadual Paulista, Botucatu Med Sch, Dept Hematol, Botucatu, SP, Brazil Univ Estadual Paulista, Botucatu Med Sch, Dept Pathol, Botucatu, SP, Brazil Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Plast Surg, Orthopedy & Surg Lab, Botucatu, SP, Brazil Univ Estadual Paulista, Postgrad Program Gen Basis Surg, Botucatu, SP, Brazil Univ Estadual Paulista, Botucatu Med Sch, Plast Surg Div, Botucatu, SP, Brazil Univ Estadual Paulista, Botucatu Med Sch, Dept Hematol, Botucatu, SP, Brazil Univ Estadual Paulista, Botucatu Med Sch, Dept Pathol, Botucatu, SP, Brazil Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Plast Surg, Orthopedy & Surg Lab, Botucatu, SP, Brazil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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