Occurrence of Yersinia enterocolitica in Raw and Pasteurized Milk

Autor: Ahmed A-H. Ahmed, Elmer H. Marth, Moustafa K. Moustafa
Rok vydání: 2019
Předmět:
Zdroj: Journal of food protection. 46(4)
ISSN: 1944-9097
Popis: Raw milk (100 samples) and pasteurized milk (100 samples) were assayed for presence of Yersinia enterocolitica , using four different enrichment and post-enrichment techniques. Isolation of Y. enterocolitica was made on MacConkey agar and Cefsulodin-Irgasan-Novobiocin (CIN) agar directly from incubated broths containing the milk, and after treatment of enrichment broths with a potassium hydroxide solution. Twelve samples of raw milk and one of pasteurized milk contained Y. enterocolitica . Nearly all isolates were found after KOH treatment. All isolates except that obtained from the pasteurized milk sample produced detectable levels of heat-stable enterotoxin. However, none of the 13 isolates agglutinated in WA-SAA, an antiserum specific for virulent Y. enterocolitica .
Databáze: OpenAIRE