The incongruent gelatinase genotype and phenotype in Enterococcus faecalis are due to shutting off the ability to respond to the gelatinase biosynthesis-activating pheromone (GBAP) quorum-sensing signal
| Autor: | Neuza Teixeira, Lynn E. Hancock, Maria de Fátima Silva Lopes, Sofia Duque Santos, Ryoji Yokohata, Pascale Serror, Jiro Nakayama, Paulo E. Marujo, Vijayalakshmi S. Iyer |
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| Přispěvatelé: | Inst Tecnol Quim & Biol, IBET, Estacao Agronom Nacl, Dept Biosci & Biotechnol, Fac Agr, Higashi Ku, Kyushu University [Fukuoka], Div Biol, Kansas State University, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Ciencia e a Tecnologia (FCT) [PDC/CVT/67270/2006, SFRH/BD/65750/2009, SFRH/BPD/14595/2003], Ciencia e a Tecnologia (FCT) through FEDER [PEst-OE/EQB/LA0004/2011], bilateral cooperation project Portugal/France (GRICES/EGIDE, Pessoa program) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Genotype GRAM-POSITIVE BACTERIA Nonsense mutation Mutant Molecular Sequence Data Microbiology Peptides Cyclic Enterococcus faecalis Microbial Pathogenicity 03 medical and health sciences Lactones Bacterial Proteins GELE BIOFILM DEVELOPMENT Gelatinase biosynthesis-activating pheromone Gelatinase Amino Acid Sequence LACTOCOCCUS-LACTIS Gene 030304 developmental biology Genetics 0303 health sciences biology Base Sequence 030306 microbiology Quorum Sensing Gene Expression Regulation Bacterial biology.organism_classification Phenotype Quorum sensing SERINE-PROTEASE Gelatinases ESCHERICHIA-COLI FSR GENES EXTRACELLULAR GELATINASE VIRULENCE SYSTEM Signal Transduction |
| Zdroj: | Microbiology-Sgm Microbiology-Sgm, 2012, 158, pp.519-528. ⟨10.1099/mic.0.055574-0⟩ |
| DOI: | 10.1099/mic.0.055574-0⟩ |
| Popis: | The concomitant presence of a complete fsr quorum-sensing system and gelE-sprE operons in Enterococcus faecalis is known to be essential for the detection of gelatinase activity. However, there are reports of the absence of gelatinase activity despite the presence of complete fsr and gelE loci. In order to understand this incongruence between genotype and phenotype we sequenced fsr and gelE loci of the E. faecalis LN68 strain, which was previously found to carry both operons but to lack gelatinase activity. Of the 59 nucleotide differences detected compared with the gelatinase-positive V583 strain, we found a nonsense mutation (a premature STOP codon) predicted to truncate the ATPase sensor domain of the FsrC protein, responsible for sensing and transducing the signal from the quorum-sensing molecule. Strain LN68 was highly affected in the expression of the gelE and sprE genes, further supporting the lack of Fsr-dependent gelE induction. When we constructed a V583 mutant with the same premature stop mutation in the fsrC gene the resulting strain was no longer able to degrade gelatin. We conclude that the reduced ability to transduce the quorum-sensing signal of the prematurely truncated FsrC protein is sufficient to explain the negative gelatinase phenotype. As the incongruent genotype and phenotype is detected in natural isolates, we believe that the silencing of the quorum-sensing system Fsr may be beneficial for some E. faecalis strains. |
| Databáze: | OpenAIRE |
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