The role of inorganic pyrophosphatase regulatory centres in subunit interactions
| Autor: | Natalia N. Vorobyeva, Tatiana I. Nazarova, Svetlana M. Avaeva |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
chemistry.chemical_classification
Pyrophosphatase Inorganic pyrophosphatase Chemistry Stereochemistry Protein subunit Dimer Biophysics Substrate (chemistry) Cell Biology Biochemistry Yeast Regulatory center Submit interaction chemistry.chemical_compound Monomer Enzyme Structural Biology Genetics Molecular Biology |
| Zdroj: | FEBS Letters. 183:387-389 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(85)80816-4 |
| Popis: | A baker's yeast inorganic pyrophosphatase derivative has been obtained in which one carboxyl group of each regulatory centre is converted to hydroxamate. This enzyme retains the capacity to hydrolyze the substrate while remaining a dimer. However, introduction of ligands to the active centre of one subunit of such enzyme does not affect the conformation of the other as is the case with native pyrophosphatase, i.e., the regulatory centre-modified enzyme behaves as a monomer. Thus, selective modification of the regulatory centres results in the disappearance of subunit interactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text