Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses
| Autor: | M. Shafiulla, Mahua Sinha, L. Appaji, Govind Babu, Linu Abraham Jacob, K C Lakshmaiah, C. S. Premalata, BK Viveka, Jayshree R. Subramanyam, Rao Cr |
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| Rok vydání: | 2016 |
| Předmět: |
medicine.disease_cause
Virus law.invention Epstein–Barr virus 03 medical and health sciences occult hepatitis B virus infection 0302 clinical medicine immune system diseases law hemic and lymphatic diseases medicine Polymerase chain reaction Hepatitis B virus business.industry non-Hodgkin lymphoma Diffuse large B-cell lymphoma medicine.disease Virology Lymphoma HBx Oncology 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health Immunology Original Article business hepatitis B virus Nested polymerase chain reaction 030215 immunology |
| Zdroj: | Indian Journal of Medical and Paediatric Oncology : Official Journal of Indian Society of Medical & Paediatric Oncology |
| ISSN: | 0975-2129 0971-5851 |
| DOI: | 10.4103/0971-5851.190353 |
| Popis: | Context: There is a need to study potential infective etiologies in lymphomas. Lymphocyte-transforming viruses can directly infect lymphocytes, disrupt normal cell functions, and promote cell division. Epstein–Barr virus (EBV) is known to be associated with several lymphomas, especially Hodgkin lymphomas (HLs). And recently, the lymphocyte-transforming role of hepatitis B virus (HBV) has been emphasized. Aims: The aim of this study was to elucidate the association of two potentially oncogenic, widely prevalent latent DNA viruses, EBV and HBV, in non-HL (NHL). Settings and Design: In this prospective study, we estimated plasma EBV and HBV DNA in NHL patients. Materials and Methods: Peripheral blood was obtained from newly diagnosed, treatment na ïve, histologically confirmed NHL patients. Plasma EBV DNA was quantified by real-time polymerase chain reaction (PCR) targeting Epstein–Barr Nucleic acid 1 while the plasma HBV DNA was detected using nested PCR targeting HBX gene. In a small subset of patients, follow-up plasma samples post-anticancer chemotherapy were available and retested for viral DNA. Results: Of the 110 NHL patients, ~79% were B-cell NHL and ~21% were T-cell NHL. Plasma EBV-DNA was detected in 10% NHLs with a higher EBV association in Burkitt lymphoma (33.3%) than other subtypes. Pretherapy HBV DNA was detected in 21% NHLs; most of them being diffuse large B-cell lymphoma (DLBCL). Moreover, 42% of DLBCL patients had HBV DNA in plasma. Since all patients were HBV surface antigen seronegative at diagnosis, baseline plasma HBV-DNAemia before chemotherapy was indicative of occult hepatitis B infection. Conclusions: Our findings indicate a significant association of HBV with newly diagnosed DLBCL. |
| Databáze: | OpenAIRE |
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