Exploring the mechanisms of graphene oxide behavioral and morphological changes in zebrafish

Autor: Diego Stéfani T. Martinez, Miriam Celi de Souza Nunes, Cláudia Vianna Maurer-Morelli, Gabriela H. da Silva, Z. Clemente, V. L. S. S. Castro, André A. de Thomaz
Přispěvatelé: ZAIRA CLEMENTE, LNNano-CNPEM, GABRIELA HELENA DA SILVA, CENA-USP, MIRIAM CELI DE SOUZA NUNES, FCM-UNICAMP, DIEGO STEFANI TEODORO MARTINEZ, CENA-USP, CLAUDIA VIANA MAURER-MORELLI, FCM-UNICAMP, ANDRE ALEXANDRE THOMAZ, FCM-UNICAMP, VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA.
Rok vydání: 2019
Předmět:
Óxido de grafeno
Embryo
Nonmammalian
Health
Toxicology and Mutagenesis
010501 environmental sciences
Ecotoxicology
01 natural sciences
Environmental impact
Behavior change
Gene expression
Zebrafish
Graphene oxide
Peixe de Água Doce
biology
Chemistry
Muscles
Aquatic environment
Gene Expression Regulation
Developmental
Embryo
General Medicine
Pollution
medicine.anatomical_structure
Larva
Toxicity
language
Acetylcholinesterase
Graphite
Locomotion
Muscle tissue
Aché
Humic acids
Andrology
medicine
Environmental Chemistry
Animals
Yolk sac
Mortality
Humic Substances
0105 earth and related environmental sciences
Danio rerio
fungi
Composto Químico
Zebrafish Proteins
biology.organism_classification
language.human_language
Matéria Orgânica
Toxidez
Biomarkers
Homeostasis
Water Pollutants
Chemical
Zdroj: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
ISSN: 1614-7499
Popis: The presence of natural organic matter such as humic acid (HA) can influence the behavior of graphene oxide (GO) in the aquatic environment. In this study, zebrafish embryos were analyzed after 5 and 7 days of exposure to GO (100 mg L-1) and HA (20 mg L?1) alone or together. The results indicated that, regardless of the presence of HA, larvae exposed to GO for 5 days showed an increase in locomotor activity, reduction in the yolk sac size, and total length and inhibition of AChE activity, but there was no difference in enzyme expression. The statistical analysis indicated that the reductions in total larval length, yolk sac size, and AChE activity in larvae exposed to GO persisted in relation to the control group, but there was a recovery of these parameters in groups also exposed to HA. Larvae exposed to GO for 7 days did not show significant differences in locomotor activity, but the RT-PCR gene expression analysis evidenced an increase in the AChE expression. Since the embryos exposed to GO showed a reduction in overall length, they were submitted to confocal microscopy and their muscle tissue configuration investigated. No changes were observed in the muscle tissue. The results indicated that HA is associated with the toxicity risk modulation by GO and that some compensatory homeostasis mechanisms may be involved in the developmental effects observed in zebrafish. Made available in DSpace on 2019-11-29T00:38:10Z (GMT). No. of bitstreams: 1 CastroExplorinGraphene2019.pdf: 1588063 bytes, checksum: 442617336ba12bcfbf2332ea5e36872c (MD5) Previous issue date: 2019
Databáze: OpenAIRE
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