Low occurrence of Bartonella in synanthropic mammals and associated ectoparasites in peri-urban areas from Central-Western and Southern Brazil

Autor: Filipe Martins Santos, Grasiela Edith de Oliveira Porfírio, Rosangela Zacarias Machado, Keyla Carstens Marques de Sousa, Shimon Harrus, Marcos Rogério André, João Bosco Vilela Campos, Ricardo Gutiérrez, Juliano Biolchi, Thiago Merighi Vieira da Silva, Gabriel Carvalho de Macedo, Daniela Pedrassani, Wanessa Teixeira Gomes Barreto, Heitor Miraglia Herrera, Luiz Ricardo Gonçalves, Darci Moraes Barros-Battesti, William de Oliveira Assis, Wesley Arruda Gimenes Nantes, Yaarit Nachum-Biala
Přispěvatelé: Universidade Estadual Paulista (Unesp), Food and Environment. The Hebrew University of Jerusalem, Dom Bosco Catholic University, University of Contestado, Federal University of Mato Grosso do Sul
Rok vydání: 2020
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 0001-706X
DOI: 10.1016/j.actatropica.2020.105513
Popis: Made available in DSpace on 2020-12-12T02:11:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-01 Universidade Estadual Paulista Worldwide, Bartonella species are known to infect a wide range of mammalian and arthropod hosts, including humans. The current study aimed to investigate the prevalence of Bartonella spp. in synanthropic mammals captured in peri-urban areas from Central-Western and Southern Brazil and their ectoparasites. For this aim, 160 mammals belonging to four species, and 218 associated arthropods were sampled. DNA was extracted and subjected to different Bartonella screening assays. Additionally, blood samples from 48 small rodents were submitted to liquid BAPGM culture followed by qPCR assay and solid culture. Two out of 55 Rattus captured in Santa Catarina state were PCR-positive for Bartonella when targeting the nuoG, 16S, and ITS loci. Sequences showed high homology with Bartonella coopersplainsensis. Conversely, all 48 small rodents, 14 capybaras and 43 opossum DNA samples from animals trapped in Mato Grosso do Sul were Bartonella negative in the HRM real time PCR assays targeting the ITS locus and gltA gene. Additionally, all mammal-associated ectoparasites showed negativity results based on HRM real time PCR assays. The present study showed, for the first time, the occurrence of B. coopersplainsensis in Brazil, shedding some light on the distribution of rats-related Bartonella in South America. In addition, the majority of rodents and marsupials were negative for Bartonella spp. Since B. coopersplainsensis reservoirs - Rattus spp. - are widely dispersed around the globe, their zoonotic potential should be further investigated. Graduate Program of Agricultural and Livestock Microbiology Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP) Department of Veterinary Pathology Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP) Koret School of Veterinary Medicine The Robert H. Smith Faculty of Agriculture Food and Environment. The Hebrew University of Jerusalem Dom Bosco Catholic University Department of Veterinary Medicine University of Contestado Federal University of Mato Grosso do Sul Graduate Program of Agricultural and Livestock Microbiology Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP) Department of Veterinary Pathology Faculty of Agrarian and Veterinary Sciences (FCAV/UNESP)
Databáze: OpenAIRE
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