Inhibition of chemokines prevents intraperitoneal adhesions in mice
| Autor: | Aydin Arici, Hakan Cakmak, Sinan Kursun, Murat Ulukus, Murat Berkkanoglu, Umit A. Kayisli, Lufang Zhang |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Chemokine Pathology Ratón medicine.medical_treatment Adhesion (medicine) Tissue Adhesions Peritoneal Diseases Peptides Cyclic Gastroenterology Mice Peritoneum Internal medicine Laparotomy medicine Animals Postoperative Period Mice Inbred BALB C biology business.industry Rehabilitation Obstetrics and Gynecology medicine.disease Effective dose (pharmacology) Cytokine medicine.anatomical_structure Reproductive Medicine biology.protein Immunohistochemistry Female Chemokines business |
| Zdroj: | Human Reproduction. 20:3047-3052 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/dei182 |
| Popis: | BACKGROUND: The present study evaluates the efficacy of a broad-spectrum chemokine inhibitor, NR58-3.14.3, in the prevention of adhesion formation after i.p. surgery in mice. METHODS: A total of 110 eight week old female Balb/c mice underwent laparotomy. Forty animals were randomly assigned to receive daily i.p. injections of either vehicle (control) or NR58-3.14.3. Time-course of adhesion formation was assessed. A titration of NR58-3.14.3 was conducted for i.p. and s.c. administrations. The effectiveness of a single intra-operative dose of NR58-3.14.3 was evaluated. Number, extent, location and type of adhesions were recorded. Immunohistochemistry of adhesions was done with leukocyte common antigen, CD45. RESULTS: Adhesion scores peaked on post-operative days 6-8. On both days 6 and 8, there were smaller adhesion size and lower cumulative adhesion scores in NR58-3.14.3-treated group. Moreover, on day 8, there were significantly fewer adhesions in NR58-3.14.3-treated group compared to controls. The least effective dose for i.p. administration of NR58-3.14.3 was 0.45 mg/animal. Subcutaneous and single intra-operative i.p. administrations were also effective in the prevention of i.p. adhesions. Although NR58-3.14.3 decreased the number of CD45 + inflammatory cells in the adhesions by 22.5% compared to control group, this was not significant. CONCLUSIONS: Our results show that this broad-spectrum chemokine inhibitor prevents post-operative adhesions in mice and may have a potential clinical use. |
| Databáze: | OpenAIRE |
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