Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells
| Autor: | Ying Cao, Lu Chen, Xiaoli Yang, Lei Fang, Lihua Shi, Min Zhang, Ning Cao, Liyang Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
PEI
polyethylenimine Protein-Arginine N-Methyltransferases PRMT1 Cellular differentiation Biochemistry Mice Neural Stem Cells neural stemness RA retinoic acid deubiquitination primNSC primitive neural stem cell cancer cell Mice Knockout Hep G2 Cells Cell cycle Neural stem cell Cell biology Neoplasm Proteins ChIP chromatin immunoprecipitation MDN1 Midasin rRNA ribosomal RNA ribosome mESC mouse embryonic stem cell Neoplastic Stem Cells Research Article Proteasome Endopeptidase Complex RT-qPCR reverse transcriptase–quantitative polymerase chain reaction neural stem cell (NSC) Biology Downregulation and upregulation WCL whole cell lysate Animals Humans IF immunofluorescence Progenitor cell NPC neural progenitor cell Molecular Biology Cell Biology Embryonic stem cell Repressor Proteins cell differentiation proteasome Proteasome A549 Cells Cancer cell USP7 tumorigenicity NSC neural stem cell PRMT protein arginine methyltransferase Ribosomes |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 1083-351X 0021-9258 |
| Popis: | Previous studies suggested that cancer cells resemble neural stem/progenitor cells in regulatory network, tumorigenicity, and differentiation potential, and that neural stemness might represent the ground or basal state of differentiation and tumorigenicity. The neural ground state is reflected in the upregulation and enrichment of basic cell machineries and developmental programs, such as cell cycle, ribosomes, proteasomes, and epigenetic factors, in cancers and in embryonic neural or neural stem cells. However, how these machineries are concertedly regulated is unclear. Here, we show that loss of neural stemness in cancer or neural stem cells via muscle-like differentiation or neuronal differentiation, respectively, caused downregulation of ribosome and proteasome components and major epigenetic factors, including PRMT1, EZH2, and LSD1. Furthermore, inhibition of PRMT1, an oncoprotein that is enriched in neural cells during embryogenesis, caused neuronal-like differentiation, downregulation of a similar set of proteins downregulated by differentiation, and alteration of subcellular distribution of ribosome and proteasome components. By contrast, PRMT1 overexpression led to an upregulation of these proteins. PRMT1 interacted with these components and protected them from degradation via recruitment of the deubiquitinase USP7, also known to promote cancer and enriched in embryonic neural cells, thereby maintaining a high level of epigenetic factors that maintain neural stemness, such as EZH2 and LSD1. Taken together, our data indicate that PRMT1 inhibition resulted in repression of cell tumorigenicity. We conclude that PRMT1 coordinates ribosome and proteasome activity to match the needs for high production and homeostasis of proteins that maintain stemness in cancer and neural stem cells. |
| Databáze: | OpenAIRE |
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