Harnessing human decellularized blood vessel matrices and cellular construct implants to promote bone healing in an ex vivo organotypic bone defect model
| Autor: | Richard O.C. Oreffo, Karl H. Schneider, Janos M. Kanczler, Heinz Redl, Stefanie Inglis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Bone Regeneration
Cell Survival Biomedical Engineering Pharmaceutical Science Biocompatible Materials 02 engineering and technology Bone healing In Vitro Techniques 010402 general chemistry Bone tissue 01 natural sciences Chorioallantoic Membrane Biomaterials Tissue engineering Osteogenesis Pregnancy medicine Human Umbilical Vein Endothelial Cells Humans Femur Bone regeneration Cells Cultured Periosteum Wound Healing Decellularization Tissue Engineering Chemistry 021001 nanoscience & nanotechnology Immunohistochemistry 0104 chemical sciences medicine.anatomical_structure cardiovascular system Blood Vessels Female 0210 nano-technology Ex vivo Biomedical engineering Blood vessel |
| Popis: | Decellularized matrices offer a beneficial substitute for biomimetic scaffolds in tissue engineering. The current study examines the potential of decellularized placental vessel sleeves (PVS) as a periosteal protective sleeve to enhance bone regeneration in embryonic day 18 chick femurs contained within the PVS and cultured organotypically over a 10 day period. The femurs are inserted into decellularized biocompatibility-tested PVS and maintained in an organotypic culture for a period of 10 days. In femurs containing decellularized PVS, a significant increase in bone volume (p < 0.001) is evident, demonstrated by microcomputed tomography (µCT) compared to femurs without PVS. Histological and immunohistological analyses reveal extensive integration of decellularized PVS with the bone periosteum, and enhanced conservation of bone architecture within the PVS. In addition, the expressions of hypoxia inducible factor-1 alpha (HIF-1α), type II collagen (COL-II), and proteoglycans are observed, indicating a possible repair mechanism via a cartilaginous stage of the bone tissue within the sleeve. The use of decellularized matrices like PVS offers a promising therapeutic strategy in surgical tissue replacement, promoting biocompatibility and architecture of the tissue as well as a factor-rich niche environment with negligible immunogenicity. |
| Databáze: | OpenAIRE |
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