Cell surface presentation of recombinant (poly-) peptides including functional T-cell epitopes by the AIDA autotransporter system
| Autor: | Martin Dipl Biol Suhr, Ingo B. Autenrieth, Annette Noll, Marc P. J. Konieczny, M. Alexander Schmidt |
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| Rok vydání: | 2000 |
| Předmět: |
Microbiology (medical)
Recombinant Fusion Proteins T-Lymphocytes Immunology Bacterial Toxins Molecular Sequence Data Epitopes T-Lymphocyte Enzyme-Linked Immunosorbent Assay Heat-labile enterotoxin medicine.disease_cause Lymphocyte Activation Microbiology Epitope Enterotoxins medicine Escherichia coli Immunology and Allergy Secretion Amino Acid Sequence Cloning Molecular Yersinia enterocolitica Adhesins Escherichia coli biology Escherichia coli Proteins Biological Transport General Medicine Chaperonin 60 biology.organism_classification Fusion protein Infectious Diseases Biochemistry Antigens Surface Autotransporter domain Bacterial outer membrane Carrier Proteins |
| Zdroj: | FEMS immunology and medical microbiology. 27(4) |
| ISSN: | 0928-8244 |
| Popis: | For the efficient surface presentation and release of virulence factors especially pathogenic Gram-negative bacteria have developed several distinct secretion mechanisms. An increasing number of pathogens in various species employs a mechanism denoted the ‘autotransporter’ pathway. This pathway is characterised by an outer membrane translocator module representing the C-terminal domain of the transported protein itself. An intriguing potential application of such systems involves the transport and surface expression of recombinant proteins or peptides, like e.g. the presentation of antigens for the generation of live oral vectors as vaccine carriers. Here we report on the incorporation of heterologous (poly-) peptides in permissive sites of the translocator module of the adhesin-involved-in-diffuse-adherence (AIDA) autotransporter system. We demonstrate the presentation of the B subunit of the heat labile enterotoxin of Escherichia coli (LTB) as well as of functional T-cell epitopes of Yersinia enterocolitica heat-shock protein 60 (Y-hsp60) on the surface of E. coli. |
| Databáze: | OpenAIRE |
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