Development of a rapid lateral flow immunoassay test for detection of exosomes previously enriched from cell culture medium and body fluids
| Autor: | María Carmen Blanco-López, Annette Paschen, Agustín Costa-García, Mar Valés-Gómez, Myriam Oliveira-Rodríguez, Eva Cernuda-Morollón, Soraya López-Martín, María Yáñez-Mó, Sheila López-Cobo, Hugh T. Reyburn |
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| Přispěvatelé: | CTQ2013-47396-R, SAF2012-32293, SAF2014-58752-R, BFU2014-55478-R (Spanish Ministry of Economy and Competitivity), FC15-GRUPIN14-022, FC-04-COF-50-MEC (Regional Government of Asturias), S2010/BMD-2326- INMUNOTHERCAN (Regional Government of Madrid), FICYT |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Histology Metastatic melanoma Medizin exosomes Biology Exosomes Exosome Rapid detection 03 medical and health sciences rapid test Lateral flow immunoassay Original Research Article in vitro diagnostics lcsh:QH573-671 lateral flow immunoassay ELISA Detection limit Rapid test lcsh:Cytology Cell Biology Molecular biology Microvesicles Cell biology 030104 developmental biology Human plasma Cell culture |
| Zdroj: | Journal of Extracellular Vesicles; Vol 5 (2016) incl supplements Journal of Extracellular Vesicles ResearcherID Journal of Extracellular Vesicles, Vol 5, Iss 0, Pp 1-10 (2016) RUO. Repositorio Institucional de la Universidad de Oviedo instname |
| Popis: | Exosomes are cell-secreted nanovesicles (40–200 nm) that represent a rich source of novel biomarkers in the diagnosis and prognosis of certain diseases. Despite the increasingly recognized relevance of these vesicles as biomarkers, their detection has been limited due in part to current technical challenges in the rapid isolation and analysis of exosomes. The complexity of the development of analytical platforms relies on the heterogeneous composition of the exosome membrane. One of the most attractive tests is the inmunochromatographic strips, which allow rapid detection by unskilled operators. We have successfully developed a novel lateral flow immunoassay (LFIA) for the detection of exosomes based on the use of tetraspanins as targets. We have applied this platform for the detection of exosomes purified from different sources: cell culture supernatants, human plasma and urine. As proof of concept, we explored the analytical potential of this LFIA platform to accurately quantify exosomes purified from a human metastatic melanoma cell line. The one-step assay can be completed in 15 min, with a limit of detection of 8.54×105 exosomes/µL when a blend of anti-CD9 and anti-CD81 were selected as capture antibodies and anti-CD63 labelled with gold nanoparticles as detection antibody. Based on our results, this platform could be well suited to be used as a rapid exosome quantification tool, with promising diagnostic applications, bearing in mind that the detection of exosomes from different sources may require adaptation of the analytical settings to their specific composition. Funding from projects CTQ2013-47396-R, SAF2012-32293, SAF2014-58752-R, BFU2014-55478-R (Spanish Ministry of Economy and Competitivity), FC15-GRUPIN14-022 (Regional Government of Asturias) and S2010/BMD-2326-INMUNOTHERCAN (Regional Government of Madrid) is acknowledged. M. Oliveira-Rodríguez thanks FICYT for her pre-doctoral grant. SLC is a recipient of an FPU fellowship from the Spanish Ministry of Education. |
| Databáze: | OpenAIRE |
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