Development of an in-house capture ELISA: An attempt to detect CagA antigen in sera of Helicobacter pylori infected patients

Autor: Esin Akcael, Zeynep Ulupinar, Barik A. Salih, Cebrail Karakus, Fahri Akbas, Yusuf Akcan, Duygu Yazici, Fatima Yucel
Přispěvatelé: AKBAŞ, FAHRİ
Rok vydání: 2020
Předmět:
0301 basic medicine
Saliva
Peptic Ulcer
medicine.drug_class
Immunology
Enzyme-Linked Immunosorbent Assay
An attempt to detect CagA antigen in sera of Helicobacter pylori infected patients.-
Journal of immunological methods
ss.112905
2020 [Salih B.
Karakus C.
Yazici D.
Ulupinar Z.
Akbas F.
Yucel F.
Akcael E.
Akcan Y.
-Development of an in-house capture ELISA]
Monoclonal antibody
digestive system
Serology
Helicobacter Infections
03 medical and health sciences
0302 clinical medicine
Antigen
Bacterial Proteins
Predictive Value of Tests
medicine
Immunology and Allergy
CagA
Humans
Serologic Tests
Antigens
Bacterial
biology
Helicobacter pylori
business.industry
Reproducibility of Results
bacterial infections and mycoses
biology.organism_classification
digestive system diseases
030104 developmental biology
Gastritis
biology.protein
medicine.symptom
Antibody
business
Biomarkers
030215 immunology
Zdroj: Journal of immunological methods. 488
ISSN: 1872-7905
Popis: The CagA protein one of the key virulence factors of Helicobacter pylori plays an important role in the pathogenesis of peptic ulcer diseases. Unfortunately the cagA gene status can only be determined by PCR while serology is an alternative approach to detect antigens or antibodies. Our aim is to detect the CagA antigen in sera of infected subjects by the development of an in-house capture ELISA test. Gastric antral biopsies and serum samples were collected from 63 patients. PCR was used to determine the cagA status. Our previously developed recombinant CagA protein and monoclonal antibody were used for setting up the capture ELISA test. H. pylori positive [(38 gastritis, 14 duodenal ulcers (DU), 11 gastric ulcer (GU)] patients were determined by PCR. The cagA gene was detected in 21 (55%) of gastritis, 11 (78%) of DU and 7 (60%) of GU patients. The reagents used in setting up the capture ELISA test following optimization displayed high performance. This study showed that our developed in-house capture ELISA has the potential to detect the CagA antigen at very low concentrations even though not detected in our H. pylori infected patients sera but we are also intended to use it in saliva and stool samples.
Databáze: OpenAIRE
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