In vitro assembly of nuclear envelope in tobacco cultured cells
| Autor: | Haruko Ueda, Kentaro Tamura, Ikuko Hara-Nishimura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
GTP'
Cell division by-2 QH426-470 confocal microscopy 03 medical and health sciences Short Article Genetics Nuclear pore 030304 developmental biology 0303 health sciences QH573-671 Chemistry Endoplasmic reticulum Vesicle 030302 biochemistry & molecular biology nucleus Lipid bilayer fusion Cell Biology nuclear envelope Plant cell Cell biology Cytosol endoplasmic reticulum Cytology Research Article |
| Zdroj: | Nucleus, Vol 12, Iss 1, Pp 82-89 (2021) Nucleus article-version (VoR) Version of Record |
| ISSN: | 1949-1042 1949-1034 |
| Popis: | The coordinated regulation of the nucelar envelope (NE) reassembly during cell division is an essential event. However, there is little information on the molecular components involved in NE assembly in plant cells. Here we developed an in vitro assay of NE assembly using tobacco BY-2 cultured cells. To start the NE assembly reaction, the demembranated nuclei and the S12 fraction (cytosol and microsomes) were mixed in the presence of GTP and ATP nucleotides. Time–course analysis indicated that tubule structures were extended from the microsomal vesicles that accumulated on the demembranated nuclei, and finally sealed the NE. Immunofluorescence confirmed that the assembled membrane contains a component of nuclear pore complex. The efficiency of the NE assembly is significantly inhibited by GTPγS that suppresses membrane fusion. This in-vitro assay system may elucidate the role of specific proteins and provide important insights into the molecular machinery of NE assembly in plant cells. |
| Databáze: | OpenAIRE |
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