Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34
| Autor: | J. H. Seol, R.M. R. Feldman, W. Zachariae, A. Shevchenko, C. C. Correll, S. Lyapina, Y. Chi, M. Galova, J. Claypool, S. Sandmeyer, K. Nasmyth, R. J. Deshaies |
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| Rok vydání: | 1999 |
| Předmět: |
F-Box-WD Repeat-Containing Protein 7
Saccharomyces cerevisiae Proteins Recombinant Fusion Proteins Ubiquitin-Protein Ligases Molecular Sequence Data Cell Cycle Proteins Biology Ubiquitin-conjugating enzyme Medical and Health Sciences Anaphase-Promoting Complex-Cyclosome Ligases SKP Cullin F-Box Protein Ligases Basic Helix-Loop-Helix Transcription Factors Genetics Animals Humans Amino Acid Sequence Cell division control protein 4 Apc11 Subunit Anaphase-Promoting Complex-Cyclosome S-Phase Kinase-Associated Proteins Ubiquitins Binding Sites F-Box Proteins Cullin Proteins Psychology and Cognitive Sciences Ubiquitin-Protein Ligase Complexes Biological Sciences Sic1 Ubiquitin ligase Enzyme Activation Biochemistry Ubiquitin-Conjugating Enzymes biology.protein CUL1 Cullin Research Paper Developmental Biology |
| Zdroj: | Seol, JH; Feldman, RMR; Zachariae, W; Shevchenko, A; Correll, CC; Lyapina, S; et al.(1999). Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes & Development, 13(12), 1614-1626. doi: 10.1101/gad.13.12.1614. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/80t4f490 |
| ISSN: | 0890-9369 |
| DOI: | 10.1101/gad.13.12.1614 |
| Popis: | SCFCdc4 (Skp1, Cdc53/cullin, F-box protein) defines a family of modular ubiquitin ligases (E3s) that regulate diverse processes including cell cycle, immune response, and development. Mass spectrometric analysis of proteins copurifying with Cdc53 identified the RING-H2 finger protein Hrt1 as a subunit of SCF. Hrt1 shows striking similarity to the Apc11 subunit of anaphase-promoting complex. Conditional inactivation of hrt1(ts) results in stabilization of the SCFCdc4 substrates Sic1 and Cln2 and cell cycle arrest at G1/S. Hrt1 assembles into recombinant SCF complexes and individually binds Cdc4, Cdc53 and Cdc34, but not Skp1. Hrt1 stimulates the E3 activity of recombinant SCF potently and enables the reconstitution of Cln2 ubiquitination by recombinant SCFGrr1. Surprisingly, SCF and the Cdc53/Hrt1 subcomplex activate autoubiquitination of Cdc34 E2 enzyme by a mechanism that does not appear to require a reactive thiol. The highly conserved human HRT1 complements the lethality of hrt1Delta, and human HRT2 binds CUL-1. We conclude that Cdc53/Hrt1 comprise a highly conserved module that serves as the functional core of a broad variety of heteromeric ubiquitin ligases. |
| Databáze: | OpenAIRE |
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