Microfluidic bioanalytical flow cells for biofilm studies: a review
| Autor: | François Paquet-Mercier, Mir Pouyan Zarabadi, Mohammad Pousti, Mehran Abbaszadeh Amirdehi, Jesse Greener |
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| Rok vydání: | 2019 |
| Předmět: |
Bioanalysis
Computer science Research areas Microfluidics 02 engineering and technology Spectrum Analysis Raman Quantitative Biology - Quantitative Methods 01 natural sciences Biochemistry Analytical Chemistry Lab-On-A-Chip Devices Electrochemistry Environmental Chemistry Quantitative Methods (q-bio.QM) Spectroscopy Microscopy Bacteria 010401 analytical chemistry Biofilm Electrochemical Techniques Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology 3. Good health 0104 chemical sciences Spectrophotometry FOS: Biological sciences Biofilms Biochemical engineering 0210 nano-technology Chemical heterogeneity |
| Zdroj: | The Analyst. 144:68-86 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c8an01526k |
| Popis: | Bacterial biofilms are among the oldest and most prevalent multicellular life forms on Earth and are increasingly relevant in research areas related to industrial fouling, medicine and biotechnology. The main hurdles to obtaining definitive experimental results include time-varying biofilm properties, structural and chemical heterogeneity, and especially their strong sensitivity to environmental cues. Therefore, in addition to judicious choice of measurement tools, a well-designed biofilm study requires strict control over experimental conditions, more so than most chemical studies. Due to excellent control over a host of physiochemical parameters, microfluidic flow cells have become indispensable in microbiological studies. Not surprisingly, the number of lab-on-chip studies focusing on biofilms and other microbiological systems with expanded analytical capabilities has expanded rapidly in the past decade. In this paper, we comprehensively review the current state of microfluidic bioanalytical research applied to bacterial biofilms and offer a perspective on new approaches that are expected to drive continued advances in this field. Comment: 16 pages, 8 figures, 2 tables |
| Databáze: | OpenAIRE |
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