Latent Membrane Protein 1 Is Dispensable for Epstein-Barr Virus Replication in Human Embryonic Kidney 293 cells
| Autor: | Elliott Kieff, Ellen Cahir-McFarland, Vicki Geiser |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
DNA Replication
Viral Diseases Chromosomes Artificial Bacterial Herpesvirus 4 Human viruses lcsh:Medicine Biology medicine.disease_cause Virus Replication Microbiology Virus Viral Matrix Proteins 03 medical and health sciences Capsid hemic and lymphatic diseases Virology medicine Humans lcsh:Science Gene 030304 developmental biology 0303 health sciences Multidisciplinary Viral matrix protein 030302 biochemistry & molecular biology HEK 293 cells lcsh:R DNA replication Epstein–Barr virus Virus Release Viral Replication 3. Good health Infectious Diseases HEK293 Cells Viral replication Viruses and Cancer Medicine lcsh:Q Gene Deletion Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 8, p e22929 (2011) |
| ISSN: | 1932-6203 |
| Popis: | Epstein Barr Virus (EBV) replicates in oral epithelial cells and gains entry to B-lymphocytes. In B-lymphocytes, EBV expresses a restricted subset of genes, the Latency III program, which converts B-lymphocytes to proliferating lymphoblasts. Latent Membrane Protein 1 (LMP1) and the other Latency III associated proteins are also expressed during virus replication. LMP1 is essential for virus replication and egress from Akata Burkitt Lymphoma cells, but a role in epithelial cell replication has not been established. Therefore, we have investigated whether LMP1 enhances EBV replication and egress from HEK293 cells, a model epithelial cell line used for EBV recombinant molecular genetics. We compared wild type (wt) and LMP1-deleted (LMP1Δ) EBV bacterial artificial chromosome (BAC) based virus replication and egress from HEK293. Following EBV immediate early Zta protein induction of EBV replication in HEK293 cells, similar levels of EBV proteins were expressed in wt- and LMP1Δ-infected HEK293 cells. LMP1 deletion did not impair EBV replication associated DNA replication, DNA encapsidation, or mature virus release. Indeed, virus from LMP1Δ-infected HEK293 cells was as infectious as EBV from wt EBV infected HEK cells. Trans-complementation with LMP1 reduced Rta expression and subsequent virus production. These data indicate that LMP1 is not required for EBV replication and egress from HEK293 cells. |
| Databáze: | OpenAIRE |
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