MiR-376a and Histone Deacetylation 9 Form A Regulatory Circuitry in Hepatocellular Carcinoma
| Autor: | Baoyue Ding, Xiaoqian Ye, Manxiang Yin, Guiqian Chen, Cheng-wen Zhang, Xinmei Zhou, Lei Yin, Huan Chen, Yongxia Zheng |
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| Rok vydání: | 2015 |
| Předmět: |
Carcinoma
Hepatocellular Physiology Down-Regulation Biology Decitabine lcsh:Physiology Histone Deacetylases Epigenesis Genetic lcsh:Biochemistry Cell Line Tumor Humans lcsh:QD415-436 Cancer epigenetics Histone deacetylase 5 lcsh:QP1-981 HDAC11 Histone deacetylase 2 HDAC10 Liver Neoplasms HDAC9 Hepatocellular Carcinoma HDAC4 Repressor Proteins MicroRNAs MiR-376a Mutation Azacitidine Cancer research Histone deacetylase |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 35, Iss 2, Pp 729-739 (2015) |
| ISSN: | 1421-9778 1015-8987 |
| DOI: | 10.1159/000369733 |
| Popis: | Background/Aims: Our previous study has demonstrated that down-regulation of miR-376a might contribute to the development of hepatocellular carcinoma (HCC), but the mechanism underlying this down-regulation remains obscure. Methods/Results: histone deacetylase (HDAC) inhibitor increased the level of miR-376a in L02 and Huh7 cells by up-regulating the acetylation level of histone 3 at the Maternally expressed 3 (Meg3) differentially methylated region (DMR). Interestingly, HDAC9, a histone deacetylase responsible for deacetylating lysine 18 of histone 3 (H3K18), was identified as the target of miR-376a. In addition, HDAC9 siRNA increased the expression of miR-376a by up-regulating the global histone H3K18 acetylation level, with Meg3 DMR included. Finally, miR-376a and HDAC9 were inversely correlated in HCC. Conclusion: HDAC9 plays an important role both as effects and targets of miR-376a. |
| Databáze: | OpenAIRE |
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