Mutations in the β-amyloid precursor protein in familial Alzheimer’s disease increase Aβ oligomer production in cellular models
| Autor: | Takahiko Tokuda, Toshiki Mizuno, Ikuko Mizuta, Yoichi Ohshima, Masaki Tanaka, Chihiro Yabe-Nishimura, Katsutoshi Taguchi, Takami Tomiyama, Fuyuki Kametani |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Mutant Article Pathogenesis 03 medical and health sciences 0302 clinical medicine Extracellular medicine Amyloid precursor protein lcsh:Social sciences (General) lcsh:Science (General) Multidisciplinary biology Chemistry Neurotoxicity Transfection medicine.disease Phenotype Cell biology 030104 developmental biology biology.protein lcsh:H1-99 030217 neurology & neurosurgery Intracellular lcsh:Q1-390 Neuroscience |
| Zdroj: | Heliyon Heliyon, Vol 4, Iss 1 (2018) |
| ISSN: | 2405-8440 |
| Popis: | Soluble oligomers of amyloid-β (Aβ) peptides (AβOs) contribute to neurotoxicity in Alzheimer’s disease (AD). However, it currently remains unknown whether an increase in AβOs is the common phenotype in cellular and animal models. Furthermore, it has not yet been established whether experimental studies conducted using models overexpressing mutant genes of the amyloid precursor protein (APP) are suitable for investigating the underlying molecular mechanism of AD. We herein employed the Flp-In™ T-REx™-293 (T-REx 293) cellular system transfected with a single copy of wild-type, Swedish-, Dutch-, or London-type APP , and quantified the levels of Aβ monomers (Aβ1-40 and Aβ1-42) and AβOs using an enzyme-linked immunosorbent assay (ELISA). The levels of extracellular AβOs were significantly higher in Dutch- and London-type APP -transfected cells than in wild-type APP -transfected cells. Increased levels were also observed in Swedish-type APP -transfected cells. On the other hand, intracellular levels of AβOs were unaltered among wild-type and mutant APP -transfected cells. Intracellular levels of Aβ monomers were undetectable, and no common abnormality was observed in their extracellular levels or ratios (Aβ1-42/Aβ1-40) among the cells examined. We herein demonstrated that increased levels of extracellular AβOs are the common phenotype in cellular models harboring different types of APP mutations. Our results suggest that extracellular AβOs play a key role in the pathogenesis of AD. |
| Databáze: | OpenAIRE |
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