Bio-prospecting of cuttle fish waste and cow dung for the production of fibrinolytic enzyme from Bacillus cereus IND5 in solid state fermentation
| Autor: | Gurupatham Devadhasan Biji, Mariadhas Valan Arasu, Eswaran Muthulakshmi, Naif Abdullah Al-Dhabi, Arumugaperumal Arun, Ponnuswamy Vijayaraghavan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Central composite design Bacillus cereus chemistry.chemical_element Environmental Science (miscellaneous) Biology 01 natural sciences Fibrinolytic enzyme 03 medical and health sciences Casein Cuttle fish waste Food science Cow dung Magnesium Substrate (chemistry) Factorial experiment biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Thrombolytic therapy 030104 developmental biology Solid state fermentation Biochemistry chemistry Solid-state fermentation Bacillus cereus IND5 Original Article 010606 plant biology & botany Biotechnology |
| Zdroj: | 3 Biotech |
| ISSN: | 2190-5738 |
| Popis: | The process parameters governing the production of fibrinolytic enzyme in solid state fermentation employing Bacillus cereus IND5 and using cuttle fish waste and cow dung substrate were optimized. The pH value of the medium, moisture content, sucrose, casein and magnesium sulfate were considered for two-level full factorial design and pH, casein and magnesium sulfate were identified as the important factors for fibrinolytic enzyme production. Central composite design was applied to investigate the interactive effect among variables (pH, casein and magnesium sulfate) and response surface plots were created to find the pinnacle of process response. The optimized levels of factors were pH 7.8, 1.1% casein and 0.1% magnesium sulfate. Enzyme production was increased 2.5-fold after statistical approach. The enzyme was purified up to a specific activity of 364.5 U/g proteins and its molecular weight was 47 kDa. It was stable at pH 8.0 and was highly active at 50 °C. The mixture of cuttle fish waste and cow dung could find great application as solid substrate for the production of fibrinolytic enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|