Urinary PGE2 in rats with chronic partial unilateral ureteral obstruction
| Autor: | Luis Salinas-Madrigal, Leslie A. Spry, George F. Steinhardt, Bashir R. Sankari |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Medullary cavity Urinary system Urine Arachidonic Acids Kidney Dinoprostone Excretion chemistry.chemical_compound Ureter Internal medicine medicine Animals Prostaglandin E2 Creatinine Arachidonic Acid business.industry Rats Inbred Strains Rats Endocrinology medicine.anatomical_structure chemistry Chronic Disease Surgery business medicine.drug Ureteral Obstruction |
| Zdroj: | The Journal of surgical research. 51(3) |
| ISSN: | 0022-4804 |
| Popis: | Urinary prostaglandin E2 (PGE2) was measured in Munich-Wistar rats with surgically created chronic partial unilateral ureteral obstruction (UUO). Mean values of bladder urine PGE2 were higher in sham than in UUO (24.5 +/- 14.4 vs 12.9 +/- 8.2 ng/mg creatinine, respectively, P less than 0.05). Following diuresis, both ureters were cannulated and urine was collected. PGE2 excretion was increased in sham (66.5 +/- 34.4 and 70.1 +/- 44.5 ng/mg creatinine, left and right, respectively). But in UUO, the obstructed kidney excreted less PGE2 than the contralateral kidney (32.1 +/- 6.0 vs 62.3 +/- 40.4 ng/mg creatinine, obstructed vs contralateral, respectively, P = 0.08). PGE2 synthesis was then determined in separated renal medullary and cortical slices. Renal medullary slices from kidneys with severe obstruction synthesized less PGE2 than the contralateral unobstructed side (3.30 +/- 1.22 vs 10.52 +/- 3.23 ng/mg wet wt-30 min, respectively, P less than 0.05) and failed to respond to arachidonic acid stimulation with any significant increase in PGE2 synthesis (3.30 +/- 1.22 vs 4.47 +/- 1.04 ng/mg wet wt-30 min, baseline vs stimulated). In contrast, contralateral unobstructed kidney slices responded with a significant increase in PGE2 synthesis (10.52 +/- 3.23 vs 21.10 +/- 2.50 ng/mg wet wt-30 min, baseline vs stimulated, P less than 0.05). We conclude that chronic partial UUO in the Munich-Wistar rats resulted in significantly less PGE2 elaboration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|