Medium-based optimization of an organic solvent-tolerant extracellular lipase from the isolated halophilic Alkalibacillus salilacus
| Autor: | Amene Samaei-Nouroozi, Mohammad Ali Faramarzi, Mohammad Reza Khoshayand, Mahmoud Doosti, Shahla Rezaei, Reza Hajihoseini, Nika Khoshnevis |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Microbiology
Substrate Specificity Nitrophenols chemistry.chemical_compound Bacterial Proteins Enzyme Stability Extracellular Response surface methodology Lipase Bacillaceae chemistry.chemical_classification Chromatography biology Chemistry Fatty Acids General Medicine Cobalt Salt Tolerance biology.organism_classification Halophile Partition coefficient Oleic acid Enzyme Alcohols biology.protein Molecular Medicine Aluminum |
| Zdroj: | Extremophiles : life under extreme conditions. 19(5) |
| ISSN: | 1433-4909 |
| Popis: | A haloalkaliphilic solvent-tolerant lipase was produced from Alkalibacillus salilacus within 48 h of growth in liquid medium. An overall 4.9-fold enhanced production was achieved over unoptimized media after medium optimization by statistical approaches. Plackett-Burman screening suggested lipase production maximally influenced by olive oil, KH2PO4, NaCl, and glucose; and response surface methodology predicted the appropriate levels of each parameter. Produced lipase was highly active and stable over broad ranges of temperature (15-65 °C), pH (4.0-11.0), and NaCl concentration (0-30 %) showing excellent thermostable, pH-stable, and halophilic properties. The enzyme was optimally active at pH 8.0 and 40 °C. Majority of cations, except some like Co(2+) and Al(3+) were positive signals for lipase activity. In addition, the presence of chemical agents and organic solvents with different log P ow was well tolerated by the enzyme. Finally, efficacy of lipase-mediated esterification of various alcohols with oleic acid in organic solvents was studied. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink