A Sequential Enzymatic Microreactor System for Ethanol Detection of Gasohol Mixtures
| Autor: | Andrea Medeiros Salgado, Belkis Valdman, Nei Pereira, Eliana Mossé Alhadeff |
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| Rok vydání: | 2005 |
| Předmět: |
Calibration curve
Bioengineering Biosensing Techniques Complex Mixtures Applied Microbiology and Biotechnology Biochemistry High-performance liquid chromatography chemistry.chemical_compound Bioreactors Phenol Molecular Biology Horseradish Peroxidase Flow injection analysis Miniaturization Chromatography Ethanol Spectrum Analysis Equipment Design General Medicine Enzymes Immobilized Dilution Equipment Failure Analysis Alcohol Oxidoreductases chemistry Flow Injection Analysis Colorimetry Gas chromatography Microreactor Gasoline Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 121:0361-0372 |
| ISSN: | 0273-2289 |
| DOI: | 10.1385/abab:121:1-3:0361 |
| Popis: | A sequential enzymatic double microreactor system with dilution line was developed for quantifying ethanol from gasohol mixtures, using a colorimetric detection method, as a new proposal to the single micro reactor system used in previous work. Alcohol oxidase (AOD) and horseradish peroxidase (HRP) immobilized on glass beads, one in each microreactor, were used with phenol and 4-aminophenazone and the red-colored product was detected with a spectrophotometer at 555 nm. Good results were obtained with the immobilization technique used for both AOD and HRP enzymes, with best retention efficiencies of 95.3 +/- 2.3% and 63.2 +/- 7.0%, respectively. The two microreactors were used to analyze extracted ethanol from gasohol blends in the range 1-30 % v/v (10.0-238.9 g ethanol/L), with and without an on-line dilution sampling line. A calibration curve was obtained in the range 0.0034-0.087 g ethanol/L working with the on-line dilution integrated to the biosensor FIA system proposed. The diluted sample concentrations were also determined by gas chromatography (GC) and high-pressure liquid chromatography (HPLC) methods and the results compared with the proposed sequential system measurements. The effect of the number of analysis performed with the same system was also investigated. |
| Databáze: | OpenAIRE |
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