The Role of Type VI Collagen in Alveolar Bone
| Autor: | Taishi Komori, Hai Pham, Priyam Jani, Sienna Perry, Yan Wang, Tina M. Kilts, Li Li, Marian F. Young |
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| Rok vydání: | 2022 |
| Předmět: |
Mice
Knockout Proteome Organic Chemistry Alveolar Bone Loss Collagen Type VI General Medicine Catalysis Computer Science Applications Inorganic Chemistry Mice Animals Physical and Theoretical Chemistry Periodontitis type VI collagen µCT analysis proteome analysis alveolar bone Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 22; Pages: 14347 |
| ISSN: | 1422-0067 |
| Popis: | Many studies have been conducted to elucidate the role of Type VI collagen in muscle and tendon, however, its role in oral tissues remains unclear. In this study, an α2(VI) deficient mouse (Col6α2-KO) model was used to examine the role of Type VI collagen in oral tissues. Tissue volume and mineral density were measured in oral tissues by µCT. Proteome analysis was performed using protein extracted from alveolar bone. In addition, alveolar bone was evaluated with a periodontitis induced model. µCT analysis showed the Col6α2-KO mice had less volume of alveolar bone, dentin and dental pulp, while the width of periodontal ligament (PDL) was greater than WT. The mineral density in alveolar bone and dentin were elevated in Col6α2-KO mice compared with WT. Our proteome analysis showed significant changes in proteins related to ECM organization and elevation of proteins associated with biomineralization in the Col6α2-KO mice. In induced periodontitis, Col6α2-KO mice had greater alveolar bone loss compared with WT. In conclusion, Type VI collagen has a role in controlling biomineralization in alveolar bone and that changes in the ECM of alveolar bone could be associated with greater bone loss due to periodontitis. |
| Databáze: | OpenAIRE |
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