In Vivo Genotoxicity and Oxidative Stress Evaluation of an Ethanolic Extract from Piquiá (Caryocar villosum) Pulp
| Autor: | Lusânia Maria Greggi Antunes, Joana D'Arc Castania Darin, Adriana Zerlotti Mercadante, Renan Campos Chisté, Mara Ribeiro Almeida, Tarsila Daysy Ursula Hermogenes Gomes, Maria de Lourdes Pires Bianchi, Alexandre Ferro Aissa |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Medicine (miscellaneous) Pharmacology medicine.disease_cause Thiobarbituric Acid Reactive Substances Mice chemistry.chemical_compound TBARS medicine Animals Humans Rats Wistar Micronucleus Tests Nutrition and Dietetics Plant Extracts Chemistry Myocardium Heart Glutathione Rats Comet assay Oxidative Stress Biochemistry Phytochemical Fruit Micronucleus test Pulp (tooth) Cattle Comet Assay NUTRIGENÔMICA Genotoxicity Oxidative stress Ericales Mutagens |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1557-7600 1096-620X |
| DOI: | 10.1089/jmf.2012.0169 |
| Popis: | In this study, the ethanolic extract obtained from piquia pulp was assessed for genotoxicity and oxidative stress by employing the micronucleus test in bone marrow and peripheral blood cells in addition to comet, thiobarbituric-acid–reactive substances (TBARS), and reduced glutathione assays in the liver, kidney, and heart. Additionally, phytochemical analyses were performed to identify and quantify the chemical constituents of the piquia extract. Wistar rats were treated by gavage with an ethanolic extract from piquia pulp (75 mg/kg body weight) for 14 days, and 24 h prior to euthanasia, they received an injection of saline or doxorubicin (15 mg/kg body weight, intraperoneally). The results demonstrated that piquia extract at the tested dose was genotoxic but not mutagenic, and it increased the TBARS levels in the heart. Further studies are required to fully elucidate how the properties of ethanolic extract of piquia pulp can affect human health. |
| Databáze: | OpenAIRE |
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