Astrocytic Ca2+ signaling is reduced during sleep and is involved in the regulation of slow wave sleep
| Autor: | Rune Enger, Gudmund Horn Hermansen, Laura Bojarskaite, Koen Vervaeke, Anna R. Chambers, Daniel M. Bjørnstad, Wannan Tang, Knut Sindre Åbjørsbråten, Klas H. Pettersen, Erlend A. Nagelhus, Rolf Sprengel, Céline Cunen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Multidisciplinary Neocortex Chemistry Whisking in animals Science General Physics and Astronomy Sleep spindle General Chemistry Sleep in non-human animals General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure medicine Wakefulness lcsh:Q Signal transduction lcsh:Science Neuroscience 030217 neurology & neurosurgery Slow-wave sleep Calcium signaling |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-16 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Astrocytic Ca2+ signaling has been intensively studied in health and disease but has not been quantified during natural sleep. Here, we employ an activity-based algorithm to assess astrocytic Ca2+ signals in the neocortex of awake and naturally sleeping mice while monitoring neuronal Ca2+ activity, brain rhythms and behavior. We show that astrocytic Ca2+ signals exhibit distinct features across the sleep-wake cycle and are reduced during sleep compared to wakefulness. Moreover, an increase in astrocytic Ca2+ signaling precedes transitions from slow wave sleep to wakefulness, with a peak upon awakening exceeding the levels during whisking and locomotion. Finally, genetic ablation of an important astrocytic Ca2+ signaling pathway impairs slow wave sleep and results in an increased number of microarousals, abnormal brain rhythms, and an increased frequency of slow wave sleep state transitions and sleep spindles. Our findings demonstrate an essential role for astrocytic Ca2+ signaling in regulating slow wave sleep. |
| Databáze: | OpenAIRE |
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