Metalloelastase is required for macrophage-mediated proteolysis and matrix invasion in mice
| Autor: | Steven D. Shapiro, J M Shipley, Robin L. Wesselschmidt, Timothy J. Ley, D K Kobayashi |
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| Rok vydání: | 1996 |
| Předmět: |
Proteolysis
Molecular Sequence Data Matrix metallopeptidase 12 Mice Inbred Strains Inflammation Biology Matrix (biology) Transfection Polymerase Chain Reaction Macrophage elastase Basement Membrane Extracellular matrix Mice Matrix Metalloproteinase 12 medicine Animals Macrophage Cloning Molecular DNA Primers Gene Library Basement membrane Multidisciplinary Base Sequence medicine.diagnostic_test Metalloendopeptidases Molecular biology Recombinant Proteins Elastin Extracellular Matrix medicine.anatomical_structure Macrophages Peritoneal medicine.symptom Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:3942-3946 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.93.9.3942 |
| Popis: | Macrophages secrete a variety of proteinases that are thought to participate in remodeling of the extracellular matrix associated with inflammatory processes. We have eliminated expression of the macrophage metalloelastase (MME) gene by targeted disruption to assess the role of this protein in macrophage-mediated proteolysis. We found that the macrophages of MME-deficient (MME-/-) mice have a markedly diminished capacity to degrade extracellular matrix components. In addition, MME-/- macrophages are essentially unable to penetrate reconstituted basement membranes in vitro and in vivo. MME is therefore required for macrophage-mediated extracellular matrix proteolysis and tissue invasion. |
| Databáze: | OpenAIRE |
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