Overfeeding with a high-concentrate diet activates the NOD1-NF-κB signalling pathway in the mammary gland of mid-lactating dairy cows
| Autor: | Wenwen Zhang, Lailai Wang, Muhammad Shahid Bilal, Yan Wang, Xiangzhen Shen, Nana Ma, Animesh Chandra Roy, Hongyu Dai |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Rumen Necrosis Lacteal Interleukin-1beta Mammary gland Cattle Diseases Inflammation Diaminopimelic Acid Microbiology Mammary Glands Animal Nod1 Signaling Adaptor Protein Internal medicine NOD1 medicine Animals Humans Lactation Nf κb signalling Interleukin-6 Tumor Necrosis Factor-alpha Chemistry NF-kappa B Interleukin Hydrogen-Ion Concentration Animal Feed Diet Gastrointestinal Tract Infectious Diseases Endocrinology medicine.anatomical_structure Gene Expression Regulation Cytokines Cattle Female medicine.symptom Acidosis Signal Transduction |
| Zdroj: | Microbial Pathogenesis. 128:390-395 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2019.01.038 |
| Popis: | Long term high-concentrate (HC) diet feeding induces subacute ruminal acidosis (SARA), which is reported to trigger a pro-inflammatory response. This study aimed to investigate the role of nucleotide-binding oligomerization domain protein 1 (NOD1) in initiating the pro-inflammatory response triggered by grain-induced SARA in the mammary gland of mid-lactating dairy cows. Twelve multiparous mid-lactating Holstein cows (455 ± 28 kg) were randomly assigned into two groups to conduct the experiment for 18 weeks as follows: one group was fed a low-concentrate (LC) diet as a control (40% grain), and the other was fed an HC diet as a treatment (60% grain). Overall, the results showed that a decreased rumen pH and elevated γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) concentrations in the HC group compared with LC group. The concentration of pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6 and tumour necrosis factor-alpha (TNF-α), significantly increased in the lacteal vein of the HC group than LC group. The mRNA expression levels of NOD1, receptor-interacting protein2 (RIP2), NF-κBp65 (p65), IL-1β, IL-6, IL-8 and TNF-α, which involved in inflammatory response, were up-regulated in the HC-induced mammary gland. The changes of the target proteins, including NOD1, p65 and pp65 presented the same tendency as those of the target genes. Collectively, long-term high concentrate feeding-induced SARA increased the rumen iE-DAP concentration which activated NOD1-NF-κB signalling pathway-dependent inflammation in the mammary gland of mid-lactating cows. |
| Databáze: | OpenAIRE |
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