Trolox enhances follicular survival after ovarian tissue autograft in squirrel monkey (Saimiri collinsi)
| Autor: | Christiani Andrade Amorim, Sarah Raphaella Rocha de Azevedo Scalercio, Danielle C. Brito, Sandro Percário, Irma C. Oskam, Sheyla Farhayldes Souza Domingues, Regiane R. Santos |
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| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty T?cnicas Reprodutivas / veterin?ria Reproductive technology Oogenesis Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Fibrosis Internal medicine Follicular phase Genetics medicine Molecular Biology Primatas / anatomia & histologia 030219 obstetrics & reproductive medicine biology Squirrel monkey medicine.disease biology.organism_classification 030104 developmental biology Transplante / veterin?ria Reproductive Medicine chemistry Saimiri / anatomia & histologia Ov?rio / anatomia & histologia Animal Science and Zoology Folliculogenesis Trolox Vasculariza??o Immunostaining Developmental Biology Biotechnology |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 1031-3613 |
| Popis: | Capes Federal University of Par?. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro Nacional de Primatas. Ananindeua, PA, Brasil. Universit? Catholique de Louvain. Institut de Recherche Exp?rimentale et Clinique. P?le de Recherche en Gyn?cologie. Brussels, Belgium. Federal University of Par?. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil. Federal University of Par?. Oxidative Stress Research Laboratory. Bel?m, PA, Brazil. Faculty of Veterinary Medicine. Oslo, Norway. Federal University of Par?. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil. Federal University of Par?. Laboratory of Wild Animal Biology and Medicine. Castanhal, PA, Brazil / Utrecht University. Faculty of Veterinary Medicine. Utrecht, The Netherlands. The aim of this study was to evaluate ovarian tissue pre-treatment with 50??M Trolox followed by heterotopic transplantation in squirrel monkeys (Saimiri collinsi) and to assess tissue functionality via immunohistochemical analysis of the stroma and ovarian follicles. Five healthy and sexually mature squirrel monkey (Saimiri collinsi) females were used. Heterotopic autografting of fresh ovarian tissue with or without previous exposure to the antioxidant Trolox was performed and grafts were recovered for analysis 7 days later. Tissue vascularisation was confirmed by both macroscopic inspection and cluster of differentiation 31 (CD31) staining. Trolox prevented massive follicular activation and kept the percentages of morphologically normal follicles higher than in untreated grafts. Expression of anti-M?llerian hormone in developing follicles was observed only in controls and Trolox-treated grafts. Also, immunostaining for growth differentiation factor-9 was positive only in primordial follicles from controls and from Trolox-treated grafts. Although Trolox improved follicular quality and avoided apoptosis in stromal cells, ovarian tissue fibrosis was increased in Trolox-treated grafts, mainly due to an increase in collagen Type I synthesis. |
| Databáze: | OpenAIRE |
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