Expression of Bcl-xL, Bax, and p53 in primary tumors and lymph node metastases in oral squamous cell carcinoma
| Autor: | Tomasz Lesniewicz, Mariusz Koda, Luiza Kanczuga-Koda, Andrzej Wincewicz, Magdalena Szymanska, M. Musiatowicz, Marek Baltaziak, Musiatowicz B, Ewa Duraj |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Pathology medicine.medical_specialty bcl-X Protein Bcl-xL General Biochemistry Genetics and Molecular Biology History and Philosophy of Science Carcinoma medicine Humans Basal cell Lymph node bcl-2-Associated X Protein biology business.industry General Neuroscience Cancer Middle Aged medicine.disease medicine.anatomical_structure Apoptosis Lymphatic Metastasis biology.protein Carcinoma Squamous Cell Immunohistochemistry Mouth Neoplasms Tumor Suppressor Protein p53 business Immunostaining |
| Zdroj: | Annals of the New York Academy of Sciences. 1090 |
| ISSN: | 0077-8923 |
| Popis: | Disturbances in expression of apoptosis-associated proteins take part in the development and progression of many human malignancies. The aim of this study was the assessment of correlations among proteins involved in apoptosis-Bcl-xL, Bax, and p53-as well as relationships of these proteins with selected clinicopathological features in oral squamous cell carcinoma. Consequently, we examined by immunohistochemistry, using the avidin-biotin-peroxidase method, Bcl-xL, Bax, and p53 expression in 56 samples of primary oral squamous cell carcinoma and in 22 matched pairs of primary and metastatic tumors. The evaluation of immunostaining of Bcl-xL, Bax, and p53 was analyzed in 10 different tumor fields, and the mean percentage of tumor cells with positive staining was evaluated. The significance of the associations was determined using Spearman correlation analysis and the chi-square test. We found positive Bcl-xL, Bax, and p53 immunostaining in 44.6%, 28.6%, and 58.9% of the studied primary tumors and in 63.6%, 45.5%, and 72.7% of lymph node metastases, respectively. Analysis of associations among studied proteins revealed positive correlation between Bcl-xL and Bax in primary tumors (P |
| Databáze: | OpenAIRE |
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