Development of biological meniscus scaffold: Decellularization method and recellularization with meniscal cell population derived from mesenchymal stem cells
| Autor: | Aylin Kara, Semra Koçtürk, Gokcen Bilici, Hasan Havitcioglu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Scaffold
Compressive Strength Population Cell Cell Culture Techniques Biomedical Engineering Meniscus (anatomy) Biomaterials 03 medical and health sciences 0302 clinical medicine Tissue engineering medicine Animals Meniscus education Cell Proliferation 030304 developmental biology 030222 orthopedics 0303 health sciences education.field_of_study Decellularization Tissue Engineering Tissue Scaffolds Chemistry Regeneration (biology) Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells musculoskeletal system Biomechanical Phenomena Extracellular Matrix body regions medicine.anatomical_structure Female Collagen Rabbits Biomarkers Biomedical engineering |
| Popis: | Tissue engineering approaches which include a combination of cells and scaffold materials provide an alternative treatment for meniscus regeneration. Decellularization and recellularization techniques are potential treatment options for transplantation. Maintenance of the ultrastructure composition of the extracellular matrix and repopulation with cells are important factors in constructing a biological scaffold and eliminating immunological reactions. The aim of the study is to develop a method to obtain biological functional meniscus scaffolds for meniscus regeneration. For this purpose, meniscus tissue was decellularized by our modified method, a combination of physical, chemical, and enzymatic methods and then recellularized with a meniscal cell population composed of fibroblasts, chondrocytes and fibrochondrocytes that obtained from mesenchymal stem cells. Decellularized and recellularized meniscus scaffolds were analysed biochemically, biomechanically and histologically. Our results revealed that cellular components of the meniscus were successfully removed by preserving collagen and GAG structures without any significant loss in biomechanical properties. Recellularization results showed that the meniscal cells were localized in the empty lacuna on the decellularized meniscus, and also well distributed and proliferated consistently during the cell culture period (p The study demonstrates that our decellularization and recellularization methods were effective to develop a biological functional meniscus scaffold and can mimic the meniscus tissue with structural and biochemical features. We predict that the obtained biological meniscus scaffolds may provide avoidance of adverse immune reactions and an appropriate microenvironment for allogeneic or xenogeneic recipients in the transplantation process. Therefore, as a promising candidate, the obtained biological meniscus scaffolds might be verified with a transplantation experiment. |
| Databáze: | OpenAIRE |
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