Preparation of an aminophenylboronic acid and N-isopropyl acrylamide copolymer functionalized stationary phase for mixed-mode chromatography
| Autor: | Lujun Wang, Yunchao Zheng, Qiurong Luo, Qin Zeng, Xiujun Ren, Zhining Xia, Die Gao, Di Zhou, Qifeng Fu, Kailian Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
Thermogravimetric analysis
Polymers Biochemistry Analytical Chemistry Polymerization Hydrolysis chemistry.chemical_compound Phenols Spectroscopy Fourier Transform Infrared Copolymer Acrylamides Chromatography Chromatography Reverse-Phase Chemistry Atom-transfer radical-polymerization Organic Chemistry Reproducibility of Results General Medicine Benzoic Acid Silicon Dioxide Boronic Acids Mixed-mode chromatography Acrylamide Thermogravimetry Theoretical plate Selectivity Hydrophobic and Hydrophilic Interactions Nuclear chemistry |
| Zdroj: | Journal of chromatography. A. 1627 |
| ISSN: | 1873-3778 |
| Popis: | A novel stationary phase co-modified with N-isopropyl acrylamide (NIPAM) and 3-aminophenylboronic acid copolymer on the silica was synthesized through atom transfer radical polymerization (ATRP) reaction for performing mixed-mode and boronate affinity chromatography. The prepared functionalized silica was characterized using Fourier transform infrared spectrometry (FT-IR), elemental analysis (EA) and thermogravimetric analysis (TGA), scanning electron micrographs (SEM) and Brunauer-Emmett-Teller (BET) measurements. The prepared column named Sil-PBA-NIPAM showed great separation performance for hydrophobic, hydrophilic, positional isomer, acidic and alkaline compounds. Besides, the mixture of cis-diol and non-cis-diol compounds was used to prove that the developed column also has potential to capture and enrich cis-diol compounds. The prepared column possesses merits of time-saving, high selectivity to cis-diol compounds and molecular-planarity selectivity compared with two commercial single-mode columns. The theoretical plates of material can reach to 57472 and the column has good hydrolysis stability and batch-to-batch reproducibility. In summary, the prepared column possesses good hydrophilicity, hydrophobicity, molecular-planarity selectivity and boronate affinity abilities for the analysis of various compounds. |
| Databáze: | OpenAIRE |
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