A novel experimental approach to evaluate guided bone regeneration (GBR) in the rat femur using a 3D-printed CAD/CAM zirconia space-maintaining barrier

Autor: Ada Codreanu, Alexandru Petre, Sergiu Drafta, Cornel Balta, Iuliana Urzica, Nicoleta Anghel-Zurbau, Sorina Dinescu, Bianca Onita-Mladin, Sami Gharbia, Simona-Rebeca Ignat, Hildegard Herman, Luminita Oancea, Andrei-Gelu Hermenean, Anca Hermenean
Rok vydání: 2021
Předmět:
0301 basic medicine
GAPDH
glyceraldehyde 3-phosphate dehydrogenase
PGA
poly(glycolic acid)
Bone tissue
Bone remodeling
0302 clinical medicine
OSX
osterix
Medicine
Bone mineral
lcsh:R5-920
Multidisciplinary
PLA
poly(lactic acid)
CAD/CAM
computer-aided design/computer-aided manufacturing
PCL
poly(e-caprolactone)
VEGF
vascular endothelial growth factor
Bone regeneration
medicine.anatomical_structure
VEGFR
vascular endothelial growth factor receptor
030220 oncology & carcinogenesis
lcsh:Medicine (General)
GBR
guided bone regeneration
PBS
phosphate-buffered saline
Bioengineering
Orthodontics
Article
Osseointegration
03 medical and health sciences
FBS
fetal bovine serum
PDGFRβ
platelet-derived growth factor receptor β
Femur
lcsh:Science (General)
Dental alveolus
ComputingMethodologies_COMPUTERGRAPHICS
Guided tissue regeneration
business.industry
IVC
individually ventilated cage
OCN
osteocalcin
030104 developmental biology
Dentistry
OPN
osteopontin
Zirconia
Implant
PFA
paraformaldehyde
business
lcsh:Q1-390
DAPI
4′
6-diamidino-2-phenylindole
ePTFE
expanded polytetrafluoroethylene
Biomedical engineering
Zdroj: Journal of Advanced Research
Journal of Advanced Research, Vol 28, Iss, Pp 221-229 (2021)
ISSN: 2090-1232
DOI: 10.1016/j.jare.2020.07.012
Popis: Graphical abstract
Introduction Obtaining a certain bone volume is an important goal in implantology or orthopedics. Thus, after tooth extraction, quite a lot of horizontal and vertical alveolar bone is lost in time and can be detrimental to the implant treatment outcome, while the treatment of critical bone defects is a considerable challenge for surgery. Objectives In this study we designed a new in vivo model as an useful experimental tool to assess guided bone regeneration (GBR) using a computer-aided design/manufacturing (CAD-CAM) space-maintaining barrier. Methods The barrier was 3D printed with three progressive heights, surgically placed on rat femur, and GBR results were analyzed at 2, 4, and 8 weeks by X-ray and bone mineral density analysis, histology/morphometry and by immunofluorescence and immunohistochemistry for osteogenesis and angiogenesis evaluation. Results The obtained results show that the proposed experimental model provides a real-time useful information on progressive bone tissue formation, which depends on the volume of isolated space created for GBR and on molecular events that lead to satisfactory vertical and horizontal bone augmentation and osteointegration. Conclusion In conclusion, the proposed customized three-dome space-maintaining barrier is suitable as an experimental tool to assess the potential of using the designed barriers in dentistry and orthopedics to promote the formation of new bone and determine their space- and time-dependent limitations. Meanwhile, guided bone augmentation for dentistry requires subsequent evaluation on an alveolar bone preclinical model followed by clinical implementation.
Databáze: OpenAIRE
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