99mTc-EC-Guanine: Synthesis, Biodistribution, and Tumor Imaging in Animals
| Autor: | Megumi Ito, Thomas Yang, Saady Kohanim, Jerry Bryant, Chang Sok Oh, Ali Azhdarinia, Vincenzo K. Wong, E. Edmund Kim, Kaoru Ozaki, Allison C. Greenwell, David J. Yang |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Biodistribution Pathology medicine.medical_specialty Guanine Pharmaceutical Science Biology Thymidine Kinase Rats Sprague-Dawley chemistry.chemical_compound Prostate Tumor Cells Cultured medicine Animals Humans Simplexvirus Tissue Distribution Pharmacology (medical) Radionuclide Imaging Lung cancer Gene Tomography Emission-Computed Single-Photon Pharmacology Cell growth Cell Cycle Organic Chemistry Prostatic Neoplasms Organotechnetium Compounds medicine.disease Molecular biology Imaging agent Rats medicine.anatomical_structure chemistry Autoradiography Molecular Medicine Radiopharmaceuticals Cell culture assays DNA Biotechnology |
| Zdroj: | Pharmaceutical Research. 22:1471-1479 |
| ISSN: | 1573-904X 0724-8741 |
| Popis: | DNA markers are useful in assessing cell proliferation. The purpose of this study was to synthesize (99m)Tc-ethylenedicysteine-guanine (EC-Guan) for evaluation of cell proliferation.Tumor cells were incubated with (99m)Tc-EC-Guan for cell cycle analysis. Prostate tumor cells that were overexpressing the HSV thymidine kinase gene, or various tumor cells were incubated with (99m)Tc-EC-Guan at 0.5-2 h. Thymidine incorporation assays were performed in lung cancer cells incubated with EC-Guan at 0.1-1 mg/well. Tissue distribution, autoradiography, and planar scintigraphy of (99m)Tc-EC-Guan and (99m)Tc-EC (control) were determined in tumor-bearing rodents at 0.5-4 h.Cell culture assays indicated that EC-Guan was incorporated in DNA, and there was no significant uptake difference between HSVTK overexpressed and normal groups. Biodistribution and scintigraphic imaging studies of (99m)Tc-EC-Guan showed increased tumor/tissue count density ratios as a function of time.Our results indicate that (99m)Tc-EC-Guan may be useful as a tumor proliferation imaging agent. |
| Databáze: | OpenAIRE |
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