Comparison of adjuvants for immune potentiating properties and side effects in mice
| Autor: | I. Claassen, P.P.A.M. Leenaars, M.A. Koedam, Eric Claassen, Coenraad F.M. Hendriksen |
|---|---|
| Přispěvatelé: | Gaubius Instituut TNO |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Male
Unclassified drug Mouse medicine.medical_treatment T-Lymphocytes Saponin Enzyme linked immunosorbent assay Immunological adjuvant medicine.disease_cause Lymphocyte Activation Mice Serous Membrane Immunostimulating agent T-cell activation Pathology T lymphocyte Evaluation Mice Inbred BALB C biology Spikoside Behavior Animal Freund adjuvant Iscom ISCOM Intraperitoneal drug administration Antibodies Bacterial Hindlimb Intestines Antibody response Female Antibody Adjuvant Injections Intraperitoneal medicine.drug Quil a Injections Subcutaneous Immunology Peritonitis Immune system Specol Antigen Adjuvants Immunologic medicine Animals Animalia Comparative Study Adjuvants Animal experiment Biology General Veterinary business.industry Animal Rabies virus Nonhuman Lactobacillus Polyclonal antibodies Subcutaneous drug administration biology.protein business Controlled study |
| Zdroj: | Veterinary Immunology and Immunopathology, 1-2, 48, 123-138 |
| Popis: | Four types of adjuvants were evaluated as alternatives to the use of Freund's complete adjuvant in mice. The adjuvants evaluated included a water-in-oil emulsion (Specol), a microorganism (Lactobacillus), preformed immune-stimulating complexes (ISCOM) containing rabies virus glycoprotein and a saponin, Quil A. The adjuvants and saline were combined with three weak immunogens (a synthetic peptide, a self antigen and a particulate antigen) and given by three different routes (intraperitoneal, subcutaneous and dorsal in the foot). The evaluation was based on clinical observations, behavioural studies, pathological lesions and capacity to support immunological responses to weak immunogens. Lesions were most severe after injection of antigen combined with Freund's adjuvant or Quil A, mild to moderate with Specol and minimal with Lactobacillus, iscom conjugates or saline. Despite pathological changes, no signs of prolonged pain or distress could be demonstrated based on clinical observations and behavioural studies. Minimal immunological responses were found after injection of antigen in combination with saline or Lactobacillus. T-cell activation and high antibody responses were found after injection of antigen-iscom conjugates or antigen in Freund's adjuvant emulsions. After Specol/antigen immunisations T-cell activation was demonstrated and high antibody titres were found except for Specol/self antigen immunisations. Presented data suggest that Specol is a possible alternative to Freund's complete adjuvant for the induction of an immune response against weak immunogens except possibly self antigens, for which preformed iscoms seem very suitable. Chemicals/CAS: Adjuvants, Immunologic; Antibodies, Bacterial |
| Databáze: | OpenAIRE |
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