An intracellular motif of GLUT4 regulates fusion of GLUT4-containing vesicles
| Autor: | Gavin I. Welsh, Sophie E Leney, Michael J.O. Wakelam, Jeremy M. Tavaré, Catherine A Heyward, Trevor R. Pettitt |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Vesicle fusion
Amino Acid Motifs Biological Transport Active Biology Transfection Membrane Fusion Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Peptide Library 3T3-L1 Cells Adipocytes Animals Insulin Phospholipase D activity lcsh:QH573-671 030304 developmental biology 0303 health sciences Glucose Transporter Type 4 Phospholipase D lcsh:Cytology Vesicle Cytoplasmic Vesicles Glucose transporter Lipid bilayer fusion Cell Biology Phosphatidic acid Cell biology Glucose chemistry Biochemistry Mutagenesis Mutation Mutant Proteins 030217 neurology & neurosurgery Intracellular Research Article |
| Zdroj: | BMC Cell Biology, Vol 9, Iss 1, p 25 (2008) BMC Cell Biology |
| ISSN: | 1471-2121 |
| Popis: | Background Insulin stimulates glucose uptake by adipocytes through increasing translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane. Fusion of GLUT4-containing vesicles at the cell surface is thought to involve phospholipase D activity, generating the signalling lipid phosphatidic acid, although the mechanism of action is not yet clear. Results Here we report the identification of a putative phosphatidic acid-binding motif in a GLUT4 intracellular loop. Mutation of this motif causes a decrease in the insulin-induced exposure of GLUT4 at the cell surface of 3T3-L1 adipocytes via an effect on vesicle fusion. Conclusion The potential phosphatidic acid-binding motif identified in this study is unique to GLUT4 among the sugar transporters, therefore this motif may provide a unique mechanism for regulating insulin-induced translocation by phospholipase D signalling. |
| Databáze: | OpenAIRE |
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