Molecular Survey on Rickettsia spp., Anaplasma phagocytophilum, Borrelia burgdorferi Sensu Lato, and Babesia spp. in Ixodes ricinus Ticks Infesting Dogs in Central Italy
Autor: | Giulia Morganti, Manuela Diaferia, Cornelia Silaghi, Gioia Capelli, Fabrizia Veronesi, Maria Teresa Antognoni, Maria Luisa Marenzoni, Stefano Gavaudan, Silvia Ravagnan, Emanuela Olivieri, Cristina Canonico |
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Přispěvatelé: | University of Zurich, Veronesi, Fabrizia |
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
10078 Institute of Parasitology
Male 0301 basic medicine Ixodes ricinus Rickettsia spp medicine.disease_cause 0302 clinical medicine 600 Technology Zoonoses Babesia spp Dog Diseases Rickettsia Pathogen 2404 Microbiology Ricinus Infectious Diseases Italy dog Female Anaplasma phagocytophilum DNA Bacterial Nymph 030231 tropical medicine 030106 microbiology Babesia 610 Medicine & health Biology Microbiology Borrelia burgdorferi sensu lato 03 medical and health sciences Dogs Borrelia burgdorferi Group Virology parasitic diseases medicine Animals Borrelia burgdorferi Ixodes 2725 Infectious Diseases DNA Protozoan bacterial infections and mycoses biology.organism_classification Tick Infestations Rickettsia helvetica 2406 Virology 570 Life sciences biology |
Popis: | Dogs are a common feeding hosts for Ixodes ricinus and may act as reservoir hosts for zoonotic tick-borne pathogens (TBPs) and as carriers of infected ticks into human settings. The aim of this work was to evaluate the presence of several selected TBPs of significant public health concern by molecular methods in I. ricinus recovered from dogs living in urban and suburban settings in central Italy. A total of 212 I. ricinus specimens were collected from the coat of domestic dogs. DNA was extracted from each specimen individually and tested for Rickettsia spp., Borrelia burgdorferi sensu lato, Babesia spp., and Anaplasma phagocytophilum, using real-time and conventional PCR protocols, followed by sequencing. Sixty-one ticks (28.8%) tested positive for TBPs; 57 samples were infected by one pathogen, while four showed coinfections. Rickettsia spp. was detected in 39 specimens (18.4%), of which 32 were identified as Rickettsia monacensis and seven as Rickettsia helvetica. Twenty-two samples (10.4%) tested positive for A. phagocytophilum; Borrelia lusitaniae and Borrelia afzelii were detected in two specimens and one specimen, respectively. One tick (0.5%) was found to be positive for Babesia venatorum (EU1). Our findings reveal the significant exposure of dogs to TBPs of public health concern and provide data on the role of dogs in the circulation of I. ricinus-borne pathogens in central Italy. |
Databáze: | OpenAIRE |
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