Differential DNA Methylation Status Between Human Preadipocytes and Mature Adipocytes
| Autor: | Chunmei Shi, Da-Ni Qin, Guan-zhong Zhu, Chenbo Ji, Chun-Mei Zhang, Xirong Guo, Lin Chen, Jin-Gai Zhu, Li Xia |
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| Rok vydání: | 2012 |
| Předmět: |
Genetics
Microarray Tumor Necrosis Factor-alpha Cellular differentiation Interleukin-8 Biophysics Cell Differentiation Cell Biology General Medicine Methylation DNA Methylation Biology Biochemistry chemistry.chemical_compound chemistry Adipocyte DNA methylation Adipocytes Humans Obesity Epigenetics Mitogen-Activated Protein Kinases Protein kinase A Gene |
| Zdroj: | Cell Biochemistry and Biophysics. 63:1-15 |
| ISSN: | 1559-0283 1085-9195 |
| DOI: | 10.1007/s12013-012-9336-3 |
| Popis: | Obesity is a multifactorial disease resulting from interactions between susceptibility genes, psychosocial, and environmental factors. However, it is becoming evident that interindividual differences in obesity susceptibility depend also on epigenetic factors, although the mechanisms have not been fully elucidated. We have undertaken a genome-wide analysis of DNA methylation of human preadipocytes and mature adipocytes to examine the differences in methylation between them. We found hypomethylation occurring in 2,701 genes and hypermethylation in 1,070 genes after differentiation. Meanwhile, Gene Ontology analysis and Ingenuity Pathway Analysis showed many significant gene functions and pathways with altered methylation status after adipocyte differentiation. In addition, Signal-Net analysis showed that tumor necrosis factor-α, mitogen-activated protein kinase, and interleukin-8 were important to the formation of this network. Our results suggest that DNA methylation mechanisms may be involved in regulating the differentiation process of human preadipocytes. |
| Databáze: | OpenAIRE |
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