Density and Polarization States of Tumor-Associated Macrophages in Human Cutaneous Squamous Cell Carcinomas Arising in Solid Organ Transplant Recipients
| Autor: | Anjela Galan, Nika Cyrus, Oscar R. Colegio, Mai-Anh Bui C, Lucinda L. Kohn, Anne Marie Rhebergen, Yao X |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Skin Neoplasms medicine.medical_treatment Cell Population Antigens Differentiation Myelomonocytic Cell Count Dermatology medicine.disease_cause 03 medical and health sciences Immunocompromised Host 0302 clinical medicine Antigens CD medicine Macrophage Humans CD40 Antigens education Aged Basement membrane Aged 80 and over education.field_of_study CD40 biology Arginase CD68 business.industry Macrophages Cell Polarity Immunosuppression General Medicine Organ Transplantation Macrophage Activation Middle Aged 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Carcinoma Squamous Cell Surgery Female Carcinogenesis business Carcinoma in Situ |
| Zdroj: | Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]. 42 |
| ISSN: | 1524-4725 |
| Popis: | BACKGROUND Solid organ transplant recipients (SOTRs) are 100 times more likely to develop cutaneous squamous cell carcinoma (SCC) with greater metastatic propensity compared with the general population, likely due to chronic immunosuppression and adverse drug effects on keratinocytes. Tumor-associated macrophages (TAMs) play critical roles in malignancies, either aiding in eradication of malignant cells or promoting tumor growth. OBJECTIVE The authors examined whether TAM density and polarization states differ between SOTRs and nontransplant individuals. MATERIALS AND METHODS The authors obtained normal skin, SCC in situ (SCCis), and SCC from SOTRs and nontransplant patients (N = 45) and stained with macrophage marker CD68, M1 marker CD40, and M2 marker arginase-1. RESULTS The authors report a significantly higher density of TAMs in both SCCis and SCC. The intratumoral macrophage infiltration in SCCis from SOTR was significantly decreased compared with nontransplant patients. Tumor-associated macrophages in SCCis and SCC displayed both M1 and M2 polarization, and M2 activation levels were significantly lower in SCC from SOTR. CONCLUSION Tumor-associated macrophages are present in early carcinogenesis and may play a critical role in the transition from SCCis to SCC, before invasion of the basement membrane by tumor cells. The intratumoral macrophage density in early stages of tumor development is significantly affected in SOTR. |
| Databáze: | OpenAIRE |
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