Identification of additional histaminergic neurons in Aplysia: Improvement of single cell isolation techniques for in tandem physiological and chemical studies
| Autor: | R.E. McCaman, J. K. Ono |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Neurons
biology Histocytochemistry General Neuroscience Histaminergic Neurotransmission biology.organism_classification Synaptic Transmission Electrophysiology chemistry.chemical_compound medicine.anatomical_structure nervous system chemistry Cellular neuroscience Aplysia medicine Animals Ganglia Neuron Neurotransmitter Neuroscience Non-spiking neuron Histamine |
| Zdroj: | Neuroscience. 5:835-840 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/0306-4522(80)90152-9 |
| Popis: | Identifiable neurons in the marine mollusc, Aplysia californica , were used to improve isolation techniques for in tandem physiological and chemical studies of the same neuron. A previous study demonstrated that the histaminergic neurons, RC-2 and LC-2, which had been subjected to intracellular recording and staining, were particularly sensitive to a freeze-substitution procedure used in isolating neurons for subsequent chemical assay. The present report describes an improvement of the freeze-substitution procedure which permits excellent recovery of histamine in physiologically manipulated C-2 neurons. The in tandem physiological and chemical procedures developed for identified neurons were used to characterize additional histamine-containing neurons which cannot be identified by visual inspection alone. These new histaminergic neurons, designated RC-3 and LC-3, are located on the ventral surface of the cerebral ganglion and are distinguishable from other adjacent histamine-containing neurons by their monosynaptic connections with some of the same follower neurons previously described for RC-2 and LC-2. The results of this study further support the role of histamine as a multiaction neurotransmitter and demonstrate the feasibility of measuring endogenous levels of various neurotransmitters in neuronal somata subsequent to intracellular recording. |
| Databáze: | OpenAIRE |
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