A dual activation mechanism for Myb-responsive genes in myelomonocytic cells
| Autor: | Karl-Heinz Klempnauer, Daniel Braas, Olesya Chayka, Pimpicha Patmasiriwat, Vichanan Yamkamon, Olga Ivanova |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
animal structures
Genes myb Biology Transfection Genes Reporter CCAAT-Enhancer-Binding Protein-alpha Animals MYB Enhancer Molecular Biology Transcription factor Gene Cell Line Transformed Reporter gene Avian Myeloblastosis Virus CCAAT-Enhancer-Binding Protein-beta fungi Promoter Cell Biology Hematology Fusion protein Molecular biology Oncogene Proteins v-myb Molecular Medicine Muramidase Hypersensitive site Chickens Transcription Factors |
| Zdroj: | Blood cells, moleculesdiseases. 40(2) |
| ISSN: | 1079-9796 |
| Popis: | The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which is responsible for the transformation of myelomonocytic cells by avian myeloblastosis virus (AMV). v-Myb is thought to exert its biological effects by deregulating the expression of specific target genes. Here we have used DNasel hypersensitive site mapping and reporter gene assays to study the activation of three Myb target genes - mini-1, the lysozyme gene and the C/EBP[ gene - all of which are activated by Myb in myelomonocytic cells but not in other hematopoietic lineages. We have found that these genes are activated by Myb via more than one cis-regulatory region. Our data suggest that all three genes are activated by Myb by dual mechanisms involving the promoters as well as enhancers. Using a cell line that expresses an estrogen-inducible v-Myb/estrogen receptor fusion protein we have also determined the effect of Myb on the expression of the C/EBP alpha gene. Our results show that C/EBP alpha expression is down-regulated by v-Myb. Thus, v-Myb affects the expression of two C/EBP family members in opposite directions. (c) 2007 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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