Comparison of vaginal microbiota in gynecologic cancer patients pre‐ and post‐radiation therapy and healthy women

Autor: Joseph W. Shelton, Konstantinos T. Konstantinidis, Yi-Juan Hu, Namita Khanna, Jinbing Bai, Lesley B. Conrad, Pretesh Patel, Isabelle Scott, Despina Tsementzi, Angela Pena-Gonzalez, Tony Y. Eng, Jessica Arluck, Mary Dolan, Deborah Watkins Bruner
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Cancer Research
medicine.medical_treatment
Physiology
postmenopausal women
Peptoniphilus
radiation therapy
0302 clinical medicine
RNA
Ribosomal
16S
Lactobacillus
Gynecologic cancer
Prevotella
Pre and post
Original Research
Radiation
biology
Middle Aged
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Peptostreptococcus
Oncology
030220 oncology & carcinogenesis
Vagina
Female
gynecologic cancer
Bacterial vaginosis
medicine.medical_specialty
food.ingredient
Genital Neoplasms
Female
lcsh:RC254-282
03 medical and health sciences
food
Internal medicine
medicine
Humans
Radiology
Nuclear Medicine and imaging
vaginal microbiota
Bacteria
Radiotherapy
business.industry
Clinical Cancer Research
Cancer
medicine.disease
biology.organism_classification
Radiation therapy
030104 developmental biology
Fusobacterium
Case-Control Studies
16S rRNA gene
business
Dysbiosis
Follow-Up Studies
Zdroj: Cancer Medicine, Vol 9, Iss 11, Pp 3714-3724 (2020)
Cancer Medicine
ISSN: 2045-7634
Popis: Background While the importance of commensal microbes in vaginal health is well appreciated, little is known about the effects of gynecological cancer (GynCa) and radiation therapy (RT) on the vaginal microbiome (VM) of postmenopausal women. Methods We studied women with GynCa, pre‐ (N = 65) and post‐RT (N = 25) and a group of healthy controls (N = 67) by sequencing the V4 region of the 16S rRNA gene from vaginal swabs and compared the diversity and composition of VMs between the three groups accounting for potential confounding factors in multivariate analysis of variance. Results Comparisons of cancer vs healthy groups revealed that Lactobacillus and Bifidobacterium have significantly higher relative abundance in the healthy group, while the cancer group was enriched in 16 phylogroups associated with bacterial vaginosis (BV) and inflammation, including Sneathia, Prevotella, Peptoniphilus, Fusobacterium, Anaerococcus, Dialister, Moryella, and Peptostreptococcus. In our sample, RT affected the α‐diversity and correlated with higher abundance of typically rare VM species, including several members of the Lacnospiraceae family, a taxon previously linked to vaginal dysbiosis. In addition to cancer and treatment modalities, age and vaginal pH were identified as significant parameters that structure the VM. Conclusions This is among the first reports identifying VM changes among postmenopausal women with cancer. RT alone seems to affect several phylogroups (12 bacterial genera), while gynecological cancer and its treatment modalities are associated with even greater significant shifts in the vaginal microbiota including the enrichment of opportunistic bacterial pathogens, which warrants further attention.
Vaginal microbial communities in postmenopausal women undergoing gynecologic cancer treatments have small but detectable differences compared to healthy controls, including higher diversity and abundance of opportunistic bacterial pathogens. Radiation therapy alone seems to affect several phylogroups (12 bacterial genera), while gynecological cancer and its treatment modalities (surgery and chemotherapy) are associated with even greater significant shifts in the vaginal microbiota including the enrichment of opportunistic bacterial pathogens, which warrants further attention.
Databáze: OpenAIRE
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