LINC01224 Promotes Colorectal Cancer Progression by Sponging miR-2467
| Autor: | Wei Chen, Lin Chen, Changjie Zhao, Qi Jiang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Immunoprecipitation Colorectal cancer proliferation LINC01224 colorectal cancer 03 medical and health sciences 0302 clinical medicine Gentamicin protection assay In vivo Pancreatic cancer medicine Luciferase Lung cancer Original Research business.industry medicine.disease invasion 030104 developmental biology Oncology Cell culture Cancer Management and Research 030220 oncology & carcinogenesis Cancer research business miR-2467 |
| Zdroj: | Cancer Management and Research |
| ISSN: | 1179-1322 |
| Popis: | Lin Chen,1 Wei Chen,1 Changjie Zhao,2 Qi Jiang1 1Department of Gastroenterology, Affiliated Dongtai Hospital of Nantong University, Dongtai, Jiangsu 224200, People’s Republic of China; 2Endoscopy Center, Affiliated Dongtai Hospital of Nantong University, Dongtai, Jiangsu 224200, People’s Republic of ChinaCorrespondence: Qi JiangDepartment of Gastroenterology, Affiliated Dongtai Hospital of Nantong University, 2 Kangfuxi Road, Dongtai, Jiangsu 224200, People’s Republic of ChinaEmail qijiang2000@21cn.comIntroduction: Colorectal cancer (CRC) is one of the most common human cancers and a leading cause of cancer-related death. Accumulating evidence has confirmed that long non-coding RNA (lncRNA) plays crucial roles in CRC development.Methods: qRT-PCR was performed to examine the expressions of LINC01224 and miR-2467. CCK-8 assay, colony formation assay and transwell invasion assay were used to examine the progression of breast cancer cells. Luciferase and RNA-binding protein immunoprecipitation (RIP) assay were applied to verify the binding site. Correlation analysis of miR-2467 and LINC01224 expression in lung cancer tissues was shown. Pancreatic cancer cells growth in vivo was evaluated using xenograft tumor assay.Results: LINC01224 expression was observed to be up-regulated in CRC tissues and cell lines. Functional studies suggested that LINC01224 silence inhibited CRC cells proliferation and invasion of CRC cells, while co-transfection with a miR-2467 inhibitor reversed these biological effects. Luciferase reporter assays illustrated that LINC01224 regulated miR-2467 directly, and RNA-binding protein immunoprecipitation (RIP) further confirmed that the suppression of LINC01224 by miR-2467 was in an RISC-dependent manner. Finally, LINC01224 silence inhibited the growth CRC cells in vivo.Conclusion: In conclusion, our findings showed that LINC01224 promoted CRC progression through sponging miR-2467. LINC01224 may serve as a potential diagnostic biomarker and therapeutic target for CRC patients.Keywords: LINC01224, miR-2467, colorectal cancer, proliferation, invasion |
| Databáze: | OpenAIRE |
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