Antioxidants Reverse the Antiproliferative Effect of High Glucose and Advanced Glycosylation End Products in Cultured Rat Mesangial Cells
| Autor: | S. Hanon, J. Prenner, S. Futterweit, H. Trachtman |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Glycation End Products
Advanced medicine.medical_specialty Taurine Antioxidant Renal glomerulus medicine.medical_treatment Biophysics In Vitro Techniques Biology Biochemistry Antioxidants chemistry.chemical_compound Internal medicine medicine Animals Vitamin E Molecular Biology Cells Cultured Mesangial cell Cell growth Cell Biology Glomerular Mesangium Rats Glucose Endocrinology chemistry Advanced glycation end-product Cell Division Bromodeoxyuridine |
| Zdroj: | Biochemical and Biophysical Research Communications. 199:346-352 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1994.1235 |
| Popis: | High glucose and elevated levels of advanced glycosylation end products (AGEs) exert an antiproliferative effect on cultured mesangial cells. In view of the role of oxygen free radicals in the pathogenesis of diabetic nephropathy, we tested whether two endogenous antioxidants, taurine and vitamin E, ameliorate the effects of an elevated ambient glucose and/or AGEs on mesangial cell growth in vitro. Regardless of whether cell proliferation was assayed by the incorporation of [3H] thymidine, direct cell counting or bromodeoxyuridine (BrdU) cell staining, both taurine and vitamin E reversed the inhibitory effect of high glucose and AGEs on mesangial cell growth. In conjunction with our previous studies indicating that taurine and vitamin E reduce collagen production in mesangial cells exposed to high glucose, these findings suggest that endogenous antioxidants attenuate diabetic glomerulosclerosis by interfering with the bioactivation of transforming growth factor-β. |
| Databáze: | OpenAIRE |
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