Mycophenolate mofetil treatment following renal transplantation decreases GTP concentrations in mononuclear leucocytes
| Autor: | Piotr Jagodziński, Sławomir Lizakowski, Ewa M. Slominska, David Goldsmith, Ryszard T. Smolenski, Bolesław Rutkowski, H. Anne Simmonds |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Purine medicine.medical_specialty Adolescent GTP' Azathioprine Pharmacology Mycophenolate Drug Administration Schedule chemistry.chemical_compound IMP Dehydrogenase Adenine nucleotide IMP dehydrogenase Humans Medicine Postoperative Period Enzyme Inhibitors Child Purine Nucleotides Aged Kidney business.industry General Medicine Middle Aged Mycophenolic Acid Kidney Transplantation Surgery Transplantation medicine.anatomical_structure chemistry Leukocytes Mononuclear Kidney Failure Chronic Female Pyrimidine Nucleotides Guanosine Triphosphate business Immunosuppressive Agents medicine.drug |
| Zdroj: | Clinical Science. 107:69-74 |
| ISSN: | 1470-8736 0143-5221 |
| Popis: | MMF (mycophenolate mofetil) has been proven to provide an effective immunosuppression by non-competitive selective reversible inhibition of IMPDH (inosine monophosphate dehydrogenase), the enzyme playing a crucial role in GTP biosynthesis. However, the exact metabolic changes induced by inhibition of IMPDH in target cells of the immune system have been the subject of recent debate. The aim of the present study was to evaluate whether MMF treatment produced sustained changes in the guanosine nucleotide pool of MNLs (mononuclear leucocytes) in vivo. Sixty-two renal failure patients were divided into three groups: chronic renal failure patients undergoing haemodialysis (CRF-HD; n=20) and two groups of patients after renal transplantation, the first on AZA (azathioprine; TN-AZA; n=23) and the second treated with MMF (TN-MMF; n=19). In addition, MNLs from 25 healthy subjects were analysed as controls. Anion-exchange HPLC was used to quantify purine and pyrimidine nucleotides in MNLs. We report a significant decrease in GTP and the total MNL guanine nucleotide pool in the TN-MMF group (P |
| Databáze: | OpenAIRE |
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