Interferon but not MxB inhibits foamy retroviruses
| Autor: | Anika Hain, Carsten Münk, Maximilian Riess, Renate König, Anna Singer, Ariane Bähr, Matthias Schweizer, Mirjam Schilling, Vanessa Serrano, Juliane Reh, Dieter Häussinger, Dirk Lindemann, Ananda Ayyappan Jaguva Vasudevan, Georg Kochs, Sumit K. Chanda, Sylvia Panitz |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Myxovirus Resistance Proteins MxA MxB Biology Viral vector Cell Line 03 medical and health sciences Interferon Virology medicine CRISPR Humans Spumavirus HEK 293 cells Restriction factor Interferon-beta Foamy viruses biology.organism_classification Molecular biology 030104 developmental biology Capsid Cell culture Ectopic expression Lentiviral vector medicine.drug |
| Zdroj: | Virology. :51-60 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2015.10.034 |
| Popis: | Foamy viruses (FV) are retroviruses that are widely distributed in primate and non-primate animal species. We tested here FV with capsids of simian and non-simian origin for sensitivity to interferon-β (IFN-β). Our data show significant inhibition of FV by IFN-β early in infection of human HOS and THP-1 but not of HEK293T cells. The post-entry restriction of FV was not mediated by the interferon-induced MxB protein that was recently identified as a capsid-interacting restriction factor targeting Human immunodeficiency virus (HIV) before integration. Neither the ectopic expression of MxA or MxB in HEK293T cells nor the lack of MxB expression in CRISPR/CAS MxB THP-1 knockout cells impacted the infection of the tested FV. IFN-β treated THP-1 and THP-1 KO MxB cells showed the same extend of restriction to FV. Together, the data demonstrate that IFN-β inhibits FV early in infection and that MxB is not a restriction factor of FV. |
| Databáze: | OpenAIRE |
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