Binding of zinc cations to human serum gamma-globulin
| Autor: | D. A. Medvedeva, E. E. Babaeva, U. A. Vorobyova, E. A. Denisova, S. B. Cheknev |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chemistry
Protein Conformation Inorganic chemistry chemistry.chemical_element Ultrafiltration General Medicine Zinc Copper General Biochemistry Genetics and Molecular Biology Globins Metal Ultrafiltration (renal) visual_art Cations visual_art.visual_art_medium γ globulin Molecule Humans Spectrophotometry Ultraviolet Uv spectrophotometry Bound copper Phenanthrolines Protein Binding |
| Zdroj: | Bulletin of experimental biology and medicine. 141(5) |
| ISSN: | 0007-4888 |
| Popis: | Binding of zinc cations to human serum gamma-globulin was studied by molecular ultrafiltration. The content of free metal in the filtrate was evaluated by reaction with o-phenanthroline. Conformation characteristics of the protein were determined by UV spectrophotometry. Our findings suggest that gamma-globulin molecule contains several zinc binding sites differing by corresponding constants and successively occupied with increase in the content of bound metal. The parameters of zinc binding correspond to those obtained in experiments with copper. Conformation status of protein with bound zinc differs significantly from that of protein with bound copper cations. |
| Databáze: | OpenAIRE |
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