Stage-specific expression of the proline-alanine transporter in the human pathogen Leishmania
| Autor: | Moshe Ephros, Doris Rentsch, Ehud Inbar, Gerald F. Späth, Dan Zilberstein, T. Liburkin-Dan, Doreen Schlisselberg, Pascale Pescher, R. Fischer-Weinberger |
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| Přispěvatelé: | Technion - Israel Institute of Technology [Haifa], Parasitologie moléculaire et Signalisation / Molecular Parasitology and Signaling, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Bern, This study was supported by grant number 1766/16 from the Israel Science Foundation., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] Protozoan Proteins 580 Plants (Botany) MESH: Phlebotomus MESH: Animals MESH: Protozoan Proteins MESH: Amino Acid Transport Systems Neutral Alanine Leishmania MESH: Proteasome Endopeptidase Complex Cell biology medicine.anatomical_structure Differentiation Leishmaniasis Visceral Intracellular Proteasome Endopeptidase Complex Proline MESH: Alanine Amino acid transport MESH: Proteolysis MESH: Insect Vectors Protein degradation Biology 03 medical and health sciences Species Specificity Lysosome parasitic diseases medicine Extracellular Animals Humans MESH: Species Specificity Amastigote Molecular Biology Signal MESH: Humans MESH: Leishmania donovani MESH: Proline 030102 biochemistry & molecular biology Intracellular parasite biology.organism_classification Stage-Specific expression Insect Vectors Amino Acid Transport Systems Neutral 030104 developmental biology MESH: Leishmaniasis Visceral Phlebotomus Proteolysis Parasitology Lysosomes Leishmania donovani MESH: Lysosomes |
| Zdroj: | Molecular and Biochemical Parasitology Molecular and Biochemical Parasitology, Elsevier, 2018, 222, pp.1-5. ⟨10.1016/j.molbiopara.2018.04.002⟩ Molecular and Biochemical Parasitology, 2018, 222, pp.1-5. ⟨10.1016/j.molbiopara.2018.04.002⟩ |
| ISSN: | 0166-6851 |
| Popis: | International audience; Leishmania are obligatory intracellular parasites that cycle between the sand fly midgut (extracellular promastigotes) and mammalian macrophage phagolysosomes (intracellular amastigotes). They have developed mechanisms of adaptation to the distinct environments of host and vector that favor utilization of both proline and alanine. LdAAP24 is the L. donovani proline-alanine transporter. It is a member of Leishmania system A that translocates neutral amino acids. Since system A is promastigote-specific, we aimed to assess whether LdAAP24 is also expressed exclusively in promastigotes. Herein, we established that upon exposing L. donovani promastigotes to amastigote differentiation signal (pH 5.5 and 37 °C), parasites rapidly and completely degrade LdAAP24 protein in both axenic and in spleen-derived amastigotes. In contrast, LdAAP24 mRNA remained unchanged throughout differentiation. Addition of either MG132 or Bafilomycin A1 partially inhibited LdAAP24 protein degradation, indicating a role for both lysosome- and proteasome-mediated degradation. This work provides the first evidence for post-translational regulation of stage-specific expression of LdAAP24. |
| Databáze: | OpenAIRE |
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