Both positional and chemical variables control in vitro proteolytic cleavage of a presenilin ortholog
| Autor: | Sibel Kalyoncu, Xingjian Tao, Hyojung Kim, Swe-Htet Naing, Alex P. Jonke, Matthew P. Torres, Volker S. Urban, Ryan C. Oliver, David M. Smalley, Raquel L. Lieberman, Josh B. George |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aspartic Acid Proteases biology Chemistry Archaeal Proteins Protein subunit Presenilins Cell Biology Cleavage (embryo) Biochemistry Presenilin 03 medical and health sciences Scissile bond Transmembrane domain 030104 developmental biology Proteolysis Amyloid precursor protein biology.protein Editors' Picks Methanomicrobiaceae Enzyme kinetics Threonine Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 293:4653-4663 |
| ISSN: | 0021-9258 |
| Popis: | Mechanistic details of intramembrane aspartyl protease (IAP) chemistry, which is central to many biological and pathogenic processes, remain largely obscure. Here, we investigated the in vitro kinetics of a microbial intramembrane aspartyl protease (mIAP) fortuitously acting on the renin substrate angiotensinogen and the C-terminal transmembrane segment of amyloid precursor protein (C100), which is cleaved by the presenilin subunit of γ-secretase, an Alzheimer disease (AD)-associated IAP. mIAP variants with substitutions in active-site and putative substrate-gating residues generally exhibit impaired, but not abolished, activity toward angiotensinogen and retain the predominant cleavage site (His–Thr). The aromatic ring, but not the hydroxyl substituent, within Tyr of the catalytic Tyr–Asp (YD) motif plays a catalytic role, and the hydrolysis reaction incorporates bulk water as in soluble aspartyl proteases. mIAP hydrolyzes the transmembrane region of C100 at two major presenilin cleavage sites, one corresponding to the AD-associated Aβ42 peptide (Ala–Thr) and the other to the non-pathogenic Aβ48 (Thr–Leu). For the former site, we observed more favorable kinetics in lipid bilayer–mimicking bicelles than in detergent solution, indicating that substrate–lipid and substrate–enzyme interactions both contribute to catalytic rates. High-resolution MS analyses across four substrates support a preference for threonine at the scissile bond. However, results from threonine-scanning mutagenesis of angiotensinogen demonstrate a competing positional preference for cleavage. Our results indicate that IAP cleavage is controlled by both positional and chemical factors, opening up new avenues for selective IAP inhibition for therapeutic interventions. |
| Databáze: | OpenAIRE |
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